Testudinate collagen and extraction method thereof
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A collagen and extraction method technology, applied in the preparation method of peptides, chemical instruments and methods, animal/human protein, etc., to achieve the effects of high purity, small equipment investment, and low process cost
Inactive Publication Date: 2014-04-23
李高霖 +1
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Problems solved by technology
However, how to extract collagen and other important components from the waste shells of marine life turtles is rarely involved and paid attention to by those skilled in the art
Method used
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Embodiment 1
[0035] The turtle collagen of the present invention is a collagen product obtained by taking turtle shells and turtle shells as raw materials, soaking, lipase enzymolysis, protease enzymolysis and separation and purification.
[0036] The above-mentioned method for extracting turtle collagen of the present invention specifically includes the following steps.
[0037] 1. Raw material pretreatment:
[0038] Use the whole tortoise shell and soft-shelled turtle shell without mildew and after drying (or drying) as the initial raw material, take 100kg of water, wash it, dry or dry it, and crush it into small pieces of raw material of 1cm ~ 2cm. The block is added to 200kg of pretreatment liquid and soaked in the temperature control tank for pretreatment. The pretreatment liquid contains NaCl and Na 2 CO 3 , the mass fraction of NaCl in the pretreatment solution is 2%, and the NaCl in the pretreatment solution 2 CO 3 The mass fraction is 15%; the temperature of soaking pretreatme...
Embodiment 2
[0048] The turtle collagen of the present invention is a collagen product obtained by taking turtle shells and turtle shells as raw materials, soaking, lipase enzymolysis, protease enzymolysis and separation and purification.
[0049] The above-mentioned method for extracting turtle collagen of the present invention specifically includes the following steps.
[0050] 1. Raw material pretreatment:
[0051] Use the whole tortoise shell and soft-shelled turtle shell (mass ratio 1:1) without mildew and dried (or dried) as the initial raw material, take 1 kg of water, clean it, dry it or dry it, and crush it into a raw material of 1 mm For small pieces, add 2kg-3kg of water, then add 1kg of vinegar to boil, cool to room temperature, filter and discard the filtrate; add the filtered 1kg of raw material small pieces to 3L of pretreatment solution and soak in a temperature-controlled tank for pretreatment , the pretreatment solution contains NaCl and Na 2 CO 3 , the mass fraction o...
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Abstract
The invention discloses testudinate collagen, which is a collagen product obtained by soaking turtle shell and / or soft-shelled turtle shell raw materials and performing lipase enzymatic hydrolysis, protease enzymatic hydrolysis, separation and purification. An extraction method for the testudinate collagen comprises the following steps of washing and crushing the pre-treated turtle and soft-shelled turtle shell raw materials, and adding the washed and crushed turtle and soft-shelled turtle shell raw materials into an acidic soaking saline solution for constant temperature soaking; and placing the turtle and soft-shelled turtle shell raw materials in lipase enzymatic hydrolysate for enzymatic hydrolysis, placing the enzymatically-hydrolyzed raw materials in protease enzymatic hydrolysate for enzymatic hydrolysis, centrifugally separating collagen liquid obtained by the enzymatic hydrolysis at high speed, and performing vacuum concentration and vacuum drying on collagen supernatant obtained by separation to obtain the collagen. The testudinate collagen product is high in purity, yield and quality, nutrient-rich and low in process cost, and contains few impurities, and the raw materials are extensive, and are fully utilized by the extraction method.
Description
technical field [0001] The invention relates to a collagen protein extracted from animal materials and an extraction method thereof, in particular to a collagen protein extracted from animal shells and other materials and an extraction method thereof. Background technique [0002] From the history of traditional Chinese medicine in my country, turtles are known as "thousand-year kings and ten thousand-year turtles". "Compendium of Materia Medica", "Chinese Pharmacopoeia of Traditional Chinese Medicine" and other historical works discuss that its function has the dual effects of treating diseases and maintaining health and prolonging life. Specifically, the shell of turtles can significantly improve the body's immunity and have a positive effect on the growth and proliferation of cells. Significant promoting effect and anti-aging effect; moreover, the shell of tortoise and turtle also has the functions of nourishing yin and nourishing kidney, nourishing blood and nourishing h...
Claims
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