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Method for isolating protein with antioxidant activity from leech freeze-dried product

A technology of antioxidant activity and leeches, which is applied in the field of protein extraction from the wide-bodied golden leeches, can solve carcinogenic and other problems, and achieve the effects of high antioxidant activity, low labor intensity of manual operation, and simplified collection procedures

Active Publication Date: 2016-05-11
浙江大学湖州市南太湖现代农业科技推广中心
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Problems solved by technology

Synthetic butylated hydroxytoluene (BHT), butyl hydroxymethoxy (BHA), propylene glycol (PG), etc. have a good effect on scavenging active oxygen free radicals, and are often used as food additives. However, recent studies have shown that BHT , BHA, PG have carcinogenic effect

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  • Method for isolating protein with antioxidant activity from leech freeze-dried product
  • Method for isolating protein with antioxidant activity from leech freeze-dried product
  • Method for isolating protein with antioxidant activity from leech freeze-dried product

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Embodiment Construction

[0013] In this example, extracting the protein from Hirudo chinensis is taken as an example. Firstly, the live Hirudomus chinensis is freeze-dried, and then pulverized by a superfine pulverizer to obtain a freeze-dried powder. Add physiological saline 15 times the volume of the freeze-dried powder to the obtained freeze-dried powder, stir evenly, put it into ice water and go through an ice bath for 6 hours. Then, the solution was put into a centrifuge and centrifuged at 8000 rpm for 15 minutes, and the supernatant was collected after precipitation. The collected supernatant was dialyzed with a 10,000 molecular weight dialysis bag and then freeze-dried to obtain the desired antioxidant activity protein.

[0014] Antioxidant activity was determined by the following method,

[0015] The first step is to measure the scavenging activity of hydroxyl radicals. Add a series of protein solutions with different concentrations of 1.0ml to the test tube, FeSO 4 (0.98mmol / l), solution 2.0...

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Abstract

The invention discloses a method of separating proteins with antioxidant activity from a leech freeze dried product. The method comprises the following steps: S1, carrying out freeze drying on living leeches; S2, carrying out superfine grinding, and taking freeze-dried powder; S3, adding normal saline which is 15 times of freeze-dried powder in volume into the leech freeze-dried powder; S4, after uniformly stirring, placing in icy water for an ice-bath for 6 hours; S5, placing the liquor in a centrifugal machine to centrifuge for 15 minutes at a speed of 8000r / m; S6, after precipitating, and collecting supernate; and S7, after dialyzing the collected supernate by a dialysis bag with molecular weight of 10000, and carrying out freeze drying. Nonhazardous proteins with high antioxidant activity can be directly extracted from leeches. The method disclosed by the invention has the advantages of simplified collecting step and low labor intensity of manual operation.

Description

technical field [0001] The invention relates to a method for isolating protein with antioxidant activity from leech freeze-dried products, in particular to a method for extracting protein from leech leech. Background technique [0002] Wide-body golden leech has a long history of being used as medicine, which was recorded in the famous Chinese medical books "Shen Nong's Materia Medica" and "Compendium of Materia Medica". , high blood pressure, coronary heart disease and tumors, etc., and can also be used for postoperative adjuvant treatment of amputated limb (finger) replantation. At the National Academic Conference for Activating Blood and Removing Blood Stasis held in 1996, leech was identified as one of the 35 Chinese medicinal materials for promoting blood circulation and removing blood stasis. At present, leech has become an indispensable raw material for Chinese and Western medicine and feed industry. There are dozens of traditional Chinese medicines as raw materials,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/14
Inventor 徐海圣尹娜陆萍彭彩娥
Owner 浙江大学湖州市南太湖现代农业科技推广中心
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