Enzymatic transesterification of lipase immobilized on a hydrophobic resin in aqueous solution
A technology for immobilizing lipase and transesterification, which can be used in hydrolase, enzyme, biochemical equipment and methods, etc., and can solve problems such as loss of catalytic performance.
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[0119] The preparation of fatty acid alkyl esters is carried out simultaneously or continuously by transesterification or esterification. In this reaction system, the activity of the biocatalyst is maintained without significant loss of activity in multiple applications, and the accumulation of glycerol and water by-products or other hydrophilic compounds on the biocatalyst is also avoided.
[0120] The present invention provides methods that utilize specific immobilized interfacial enzymes that retain high activity and stability during many production cycles. In particular, lipase and phospholipase preparations are used in transesterification / esterification reactions. These reactions can be used to make food, cosmetics, and biofuels ("biodiesel"). Of particular interest, these enzymes can be used to synthesize fatty acid short-chain alkyl esters for use as "biodiesel".
[0121] The present invention utilizes stable immobilized interfacial enzymes with high tolerance to short-cha...
example
[0148] Summary
[0149] All experiments were performed either in a 30 ml glass tube with a glass filter in the center at the bottom, or a 500 ml mechanically stirred reactor with a sintered glass filter with a porosity of 150-250 μm at the bottom. A typical reaction medium contains a fatty acid source, alcohol (usually methanol or ethanol) on a 1:1 molar basis relative to the fatty acid (whether it is free or bound to the glycerol backbone) (for free fatty acids and monoglycerides, 1:1 , It is 1:2 for diglycerides and 1:3 for triglycerides, which is good for alcohol). The fatty acid source is premixed with varying amounts of alkaline buffer (in a specific embodiment, sodium bicarbonate). The reaction is initiated by adding lipase (10-15% by weight) immobilized on a hydrophobic resin, and the reaction medium is either mechanically shaken or stirred at 30°C. Unless otherwise indicated, the amount of alcohol is added equally in three steps, each with an interval of 1 hour. The re...
example 1
[0153] Fixed to Amberlite as a hydrophobic resin R XAD1600 and Duolite as a hydrophilic resin R Lipase derived from Thermomyces lanuginosa on D568 and Sepabeads as hydrophobic resin R Transesterification activity of Pseudomonas-derived lipase on SP70 and porous silica as a hydrophilic resin.
[0154] Reaction conditions: refined and bleached soybean oil (20 g) contains 1% by weight 0.1M sodium bicarbonate solution. Methanol (2.5 ml) was gradually added in three equivalent batches, each with an interval of 1 hour. The reaction medium containing 10% by weight of the lipase preparation was shaken at 300 rpm and 30°C. The results are shown in figure 1 in.
[0155] figure 1 The results shown show that using the same batch of enzymes in the first 5 cycles in the presence of 1% by weight sodium bicarbonate solution, the thermophilic hyphomyces pilosides and pseudomonas immobilized on different resins The lipases of the genus bacteria all show high transesterification activity. It was ...
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