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Method for extracting and preparing low molecular fucoidan from Ascophyllum mackaii

A technology of molecular fucoidan sulfate and fucoidan sulfate, applied in the field of seaweed chemistry, can solve the problems of complex composition and no standard method, etc.

Inactive Publication Date: 2014-05-14
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Liu Hongying et al. and Li Zhaoxin et al. have conducted some discussions on the content determination of fucoidan sulfate, there is no standard method due to its complex composition.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Weigh 100 kg of dry Ascophyllum nodosum or giant algae from South Africa, Chile, Peru, etc., cut into 1-2cm small pieces, transfer to a stainless steel stirrer with temperature regulation and layered outlet, add 900 kg of 0.1% mol / L of hydrochloric acid solution, adjust the temperature to 60 ° C, stir and extract for 4 hours. After standing for 2 hours, open the discharge port to collect the bottom layer of Ascophyllum nodosum solid, the upper layer of floating matter and the dilute acid extract respectively. Continue to add 300 kg of water to the bottom layer of Ascophyllum nodosum solid, soak and stir for 4 hours, centrifuge at 1000r / min, continue to retain the solid algae residue and collect the water extract. Combine the above acid extract and water extract.

[0023] The upper floating matter was adjusted to pH 5.5 with NaOH, filtered through a 100-mesh silk screen, and the filtrate A was collected. The solid algae residue extracted from the above dilute acid and...

Embodiment 2

[0025] Weigh 100 kg of dry Ascophyllum nodosum or giant algae from South Africa, Chile, Peru, etc., cut into 1-2cm small pieces, transfer to a stainless steel stirrer with temperature regulation and layered outlet, add 600 kg of 0.2% mol / L of hydrochloric acid solution, adjust the temperature to 40°C, stir and extract for 1.3h. After standing for 3 hours, the outlet was opened to collect the bottom layer of Ascophyllum nodosum solid, the upper layer of floating matter and the dilute acid extract respectively.

[0026] Continue to add 500 kg of water to the bottom layer of Ascophyllum nodosum solid, soak and stir for 4 hours, centrifuge at 1000r / min, continue to retain the solid algae residue and collect the water extract.

[0027] Combine the above acid extract and water extract.

[0028] The upper floating matter was adjusted to pH 6.5 with NaOH, filtered through a 100-mesh silk screen, and the filtrate A was collected. The solid algae residue extracted by the above dilute...

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Abstract

The invention relates to the alga chemical field, and concretely relates to a method for extracting and preparing low molecular fucoidan from Ascophyllum mackaii. The method is characterized in that Ascophyllum mackaii is immersed and extracted by employing dilute hydrochloric acid and water, a soak solution is used for purifying an extract through a foaming machine, an upper layer floater and a lower layer sediment are respectively collected, and polysaccharide in the Ascophyllum mackaii is fully extracted and collected by using a filtering collection and a cell disruption extraction method. An ultrafiltration technology is used for removing salinity and the graded products in 20000 Dalton can be obtained. The fucoidan crude product is obtained by an ethanol precipitation method. Purification and classification of an anion exchange resin chromatographic column of DEAE-Sepharose F.F. can be carried out. Next classification and concentration of an 8-14KD dialysis bag are carried out, and cryoconcentration and freeze drying are carried out to obtain the fucoidan product.

Description

technical field [0001] The invention relates to the field of seaweed chemistry, in particular to a method for extracting and preparing low-molecular-weight fucoidan sulfate from Ascophyllum nodosum. Background technique [0002] Fucoidan, also known as fucoidan or fucoidan, is a water-soluble sulfated heteropolysaccharide commonly found in brown algae and some echinoderms. Studies have found that fucoidan sulfate has various biological activities such as anticoagulation, anti-oxidation, anti-tumor, and anti-virus, and with the continuous improvement of its application value, the demand is also increasing. Therefore, how to improve the extraction rate of the main components in fucoidan sulfate is the key to its industrial utilization. [0003] In addition to L-fucose and sulfate, the main components of fucoidan sulfate also contain galactose, xylose, mannose, glucose, uronic acid, protein, etc. More and more studies have shown that its activity It is closely related to the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 韩丽君袁毅郭书举曲桂艳刘旭
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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