A method of artificially inoculating tobacco bacterial wilt with isolated leaf veins

Abstract

The invention discloses an artificial infection method using in-vitro leaf vein slices to inoculate tobacco bacterial wilt. The method comprises the following steps: (1) preparing a main appliance for inoculating; (2) cultivating tobacco seedlings; (3) preparing a bacterium solution for inoculating; (4) collecting and treating the in-vitro leaf vein slices; (5) placing the in-vitro leaf vein slices on an inoculating appliance; (6) inoculating bacteria; (7) treating after inoculating. The artificial infection method has the advantages that 1, the inoculating infection process has short time and few occupied space field; the method is simple, easy and convenient; technical operation with high difficulty is not needed and the working efficiency is high; 2, large tobacco leaves are used so as to easily reflect the difference of result disease conditions and the tobacco leaves are easy to obtain; a disease symptom is consistent to a field symptom and is clear and easy to identify; the repeatability is good; the partial condition of the inoculating infection is easy to control and the standardization of a technical method is easy to realize.

Description

technical field [0001] The present invention relates to agricultural technology and biotechnology. Specifically, it is an artificial pathogenic method for inoculating tobacco bacterial wilt with isolated leaf veins. Background technique [0002] In the study of plant disease control and prevention, the research on many basic issues or important theoretical issues cannot be separated from the basic means of artificial inoculation. The artificial disease of plant diseases can be inoculated on living plants, and can also be inoculated on isolated plant organ materials. Although the research results of using living plants to inoculate the pathogenesis can better reflect the original appearance of the host-bacteria interaction, inoculation on living organisms often requires more time and space, and consumes more energy and financial resources. However, it is difficult to control local conditions; while the use of isolated plant organ materials to inoculate the disease often req...

AI Technical Summary

Problems solved by technology

Although the inoculation method system is very effective, there are also some obvious disadvantages: 1) The operation technique is difficult, and the bacterial solution must be accurately pressed or injected into the thinner tobacco leaf tissue whose thickness is almost equal to the diameter of the needle. Well-trained operators are required, and the operation efficiency is relatively low; 2) The result of inoculation is mainly manifested as local necrosis of the tissue near the inoculation point, which has a certain gap with the systemic infection symptoms of the disease

In the high-paced contemporary work, it is often required that the applied technical methods are simple and convenient to work with high efficiency, high throughput and high accuracy. The above-mentioned disadvantages obviously make it difficult for the current in vitro tobacco inoculation method to meet the requirements.

[0005] The main symptom of tobacco bacterial wilt is wilting, and it can also cause necrosis of the conduction system and its surrounding tissues. The conduction tissue of the leaf is distributed on the leaf veins, and the necrosis of the conduction system and its surrounding tissues can lead to changes in the appearance of the leaf veins. Brown to black necrosis, this symptom feature should be used in artificial inoculation, but so far there is no such clear symptom, applied in the practice of artificial inoculation and supporting technology established

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