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A Pseudomonas stutzeri Strain Efficiently Treating Nitrogenous Sewage and Its Application

A technology of Pseudomonas stutzeri and nitrifying bacteria, applied in biological water/sewage treatment, water/sludge/sewage treatment, bacteria, etc.

Active Publication Date: 2016-01-20
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the secondary effluent of urban sewage contains a certain amount of nutrients such as nitrogen and phosphorus, which directly replenish urban landscape water bodies, rivers and lakes, there is a risk of eutrophication of water bodies

Method used

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  • A Pseudomonas stutzeri Strain Efficiently Treating Nitrogenous Sewage and Its Application
  • A Pseudomonas stutzeri Strain Efficiently Treating Nitrogenous Sewage and Its Application
  • A Pseudomonas stutzeri Strain Efficiently Treating Nitrogenous Sewage and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Isolation and identification of Pseudomonas stutzeri DN-LWX19

[0040] 1. Isolation of Pseudomonas stutzeri DN-LWX19

[0041] Put 10 filter materials at the outlet of the denitrification filter (see Table 1 for the water quality on the day of sampling) into 40 mL of enrichment medium (1 liter of liquid prepared with 5 g of beef extract, 10 g of peptone, 1 g of potassium nitrate and water, pH Cultivate overnight at 30°C in a 100mL Erlenmeyer flask (7.0-7.2), let the microorganisms on the filter material disperse, enrich and grow, draw 1mL of bacterial suspension into a centrifuge tube filled with 9mL sterile water, and obtain a dilution of 10 -1 According to this method, the bacterial suspension was diluted with sterile water to 10 -15 , to obtain a series of dilution gradient bacterial suspension. Pipette 0.1mL dilution 10 -8 -10 -15 Bacteria suspension to denitrifying bacteria isolation medium (1 liter of liquid prepared with 5g of beef extract, 10g of p...

Embodiment 2

[0067] Embodiment 2, the preparation of denitrifying bacteria agent

[0068] Put the slant medium (each liter of slant medium is made into 1 liter of liquid with beef extract 5g, peptone 10g, potassium nitrate 1g, agar 18g and water; pH is 7.0-7.2; sterilized at 121°C for 20 minutes) For the preserved Pseudomonas stutzeri (Pseudomonasstutzeri) DN-LWX19CGMCCNo.8521, use a sterilized inoculation loop to pick a loop and inoculate it into 40 mL of enrichment medium (5g of beef extract and 10g of peptone for each liter of enrichment medium) , Potassium Nitrate 1g, Potassium Dihydrogen Phosphate 0.4g and water to prepare 1 liter of liquid, pH is 7.0-7.2; sterilized at 121°C for 20 minutes) in a 100mL Erlenmeyer flask, shaken at 30°C for 12h, The obtained bacterial liquid is the denitrifying bacterial agent, and the content of Pseudomonas stutzeri (Pseudomonasstutzeri) DN-LWX19CGMCCNo.8521 in the denitrifying bacterial agent is 2×10 10 cfu / mL.

Embodiment 3

[0069] Example 3. Denitrification effect of Pseudomonasstutzeri DN-LWX19CGMCCNo.8521 in simulated sewage

[0070] Sewage is taken from SBR reactor effluent (NO 3 - -N32mg / L, NO 2 --N4.5mg / L, COD50mg / L), the carbon-nitrogen ratio is about 1.56:1. In order to meet the carbon-to-nitrogen ratio required for denitrification, sodium acetate was added to the wastewater until the concentration of COD was 275mg / L to obtain simulated wastewater. The NO of the simulated sewage 3 - -N concentration is 32mg / L, NO 2 - -N concentration is 4.5mg / L, COD concentration is 275mg / L.

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Abstract

The invention discloses pseudomonas stutzeri for efficiently processing nitrogen-containing sewage and an application thereof. The pseudomonas stutzeri is pseudomonas stutzeri DN-LWX19; and a preservation number of the pseudomonas stutzeri in General Microbiology Centre of Microbial Preservation Management Committee in China is CGMCC No.8521. Through the pseudomonas stutzeri DN-LWX19, a NO3<-1>-N removal rate in 24 hours in simulation sewage with a NO3<-1>-N concentration of 32mg / L can reach 98%, the NO3<-1>-N removal rate in 32 hours can reach 100% and no nitrite is accumulated; and a consumption ratio of carbon to nitrogen after achieving complete denitrification in 32 hours is 4.4:1.

Description

technical field [0001] The invention relates to a strain of Pseudomonas stutzeri for efficiently treating nitrogen-containing sewage and its application. Background technique [0002] Sewage recycling is an effective way to alleviate the shortage of water resources, prevent and control water environmental pollution, and improve the quality of water environment. However, since the secondary effluent of urban sewage contains a certain amount of nutrients such as nitrogen and phosphorus, which directly replenish urban landscape water bodies, rivers and lakes, there is a risk of eutrophication of water bodies. The current denitrification technology is still the most efficient and widely used biological denitrification technology. [0003] The conversion process of nitrogen in traditional biological denitrification includes ammonification, nitrification and denitrification. The denitrification process is the process of converting nitrogen into gaseous nitrogen and finally remov...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/38
Inventor 孙迎雪廉巍雪周晓黎田媛
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY