Process method for refining neutral protease from tussah cocoon shell

The technology of a neutral protease and a process method is applied in the field of the neutral protease refining process of tussah cocoon shells, which can solve the problems of failing to achieve ecology, environmental protection and clean production, environmental pollution, etc., and achieve social benefits, environmental improvement, and fiber fluffy. Soft and shiny effect

Inactive Publication Date: 2014-05-14
丹东中天生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, this degumming method only makes a certain contribution to the degumming effect and reduces energy consumption. Due to the use of lye,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0028] Example 1

[0029] (1) Pretreatment

[0030] Tussah cocoon shells are manually removed from impurities and impurities, and permeated according to the positive pressure method. The pretreatment process of the positive pressure method has been applied for an invention patent in another case. Put 24 kilograms of tussah cocoon shells and 240 kilograms of water into the high-temperature and high-pressure tank. The temperature is increased, and the steam pressure is maintained at 0.10 MPa for 40 minutes. The pressure is reduced to normal pressure. The high-temperature and high-pressure tank is opened to take the cocoon shells and cool to normal temperature.

[0031] Rinse: Rinse the pretreated tussah cocoon shell with water for 5 min at room temperature.

[0032] The processing time of the positive pressure method is determined by the steam pressure. The higher the steam pressure, the shorter the processing time. When it exceeds 0.25 MPa, the processing time has little effect on th...

Example Embodiment

[0038] Example 2

[0039] (1) Pretreatment

[0040] The tussah cocoon shell is manually removed from impurities. Put 24 kg of tussah cocoon shells in a high temperature and high pressure tank, 240 kg of water, heat up, maintain the steam pressure of 0.20 MPa for 50 minutes, reduce the pressure to normal pressure, and open the high temperature and high pressure tank to take 岀柞 Cocoon shell, cooled to room temperature.

[0041] Rinse: Take the pretreated tussah cocoon shell and rinse with water for 8 min at room temperature.

[0042] (2) Protease degumming

[0043] Put 48 kg of pretreated tussah cocoon shells, add 580 kg of water, put 50,000 units of neutral protease (Shandong Weifang Sukehan Biological Co., Ltd.) 2.9 kg, water temperature 40~45℃, pH value 6.8, time 120min, test silk The residual glue rate is 3.8%, stop degumming.

[0044] The residual glue rate is 3.8%, the residual oil rate is 0.21, and the cleanliness is 90.

[0045] Others are the same as in Example 1.

Example Embodiment

[0046] Example 3

[0047] (1) Pretreatment

[0048] The tussah cocoon shells are manually removed from impurities, and the permeation treatment is carried out according to the positive pressure method. Put 24 kilograms of tussah cocoon shells and 264 kilograms of water into a high temperature and high pressure tank. The temperature is increased, and the steam pressure is maintained at 0.25 MPa for 60 minutes, and the pressure is reduced to normal pressure. , Open the high-temperature and high-pressure tank to take the cocoon shell of the cocoon, and cool it to room temperature.

[0049] Rinse: Take the pre-treated tussah cocoon shell and rinse with water for 10 minutes at room temperature.

[0050] (2) Protease degumming

[0051] Put in 48 kg of pretreated tussah cocoon shells, add 720 kg of water, put in 50,000 units of neutral protease (Shandong Weifang Sukehan Biological Co., Ltd.) 3.36 kg, water temperature 45°C, pH value 7.5, time 60min, and detect silk residue The glue rate is ...

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PUM

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Abstract

The invention relates to a process method for refining neutral protease from a tussah cocoon shell. The process method comprises the following steps of pretreatment, wherein a positive-pressure pretreatment process or a soap-alkaline pretreatment process is adopted; and neutral protease degumming, wherein 100 parts of pretreated tussah cocoon shells are prepared, the bath ratio is 1:10-15, 5-7 parts of the neutral protease (5 million units) are used, the water temperature is 40-45 DEG C, pH value is 6.5-7.5, and degumming is finished until the residual gum content of the silk fibers is detected to be 3-5%. Sericin peptide bonds of silk are removed by being broken into amino acids and polypeptide through hydrolysis in the presence of protease, fibroin is not influenced, particularly the pretreatment adopts a high-temperature and high-pressure steaming mode, the energy is saved, no chemicals contamination is caused, the refined waste silk has large tearing strength, and the fibers are fluffy, soft, glossy and have no raw and smooth cocoons; the degummed sewage water contains sericin and little neutral protease preparation, and is dehydrated through sedimentation of protein flocculating agents, and then drying is carried out to obtain sericin, and the sericin can be coarsely processed into organic fertilizers, so that the environment is improved and the social benefit is increased.

Description

technical field [0001] The invention relates to a process for degumming tussah cocoon shells, in particular to a process for degumming with neutral protease. Background technique [0002] Tussah silk is composed of sericin and silk fibroin, sericin wraps silk fibroin, silk fibroin accounts for 84%, sericin accounts for about 12%~16%, contains about 2.5% waxy pigments and inorganic substances, sericin, silk Silk fibroin is composed of protein and amino acids. Sericin is wrapped in the outer layer of silk fibroin. Normally silk spinning and weaving need to remove sericin. This degumming process is called refining. The most traditional refining method is the soap-alkali method. The principle of chemical degumming with soap is to remove sericin by utilizing the difference in stability and sensitivity of sericin and silk fibroin to alkali. Sericin is easier to be hydrolyzed and dissolved in water than silk fibroin. Removed by washing. The traditional degumming process with soap...

Claims

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Application Information

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IPC IPC(8): D01C3/02
Inventor 慕德明王宝贤徐友兰张少华
Owner 丹东中天生物科技有限公司
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