Method for detecting content of casein phosphopeptides in dairy product
A casein phosphopeptide and protein phosphopeptide technology, applied in the field of detection, can solve the problems of cumbersome operation steps, many interference factors, and long time consumption, and achieve the effects of high detection accuracy, simplified operation steps, and shortened detection time
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Embodiment 1
[0042] This embodiment provides a method for detecting casein phosphopeptide in dairy products, which includes the following steps:
[0043] A. Purification of CPP raw materials
[0044] Weigh 10g of CPP raw material into a 50mL beaker, dissolve it with pure water, then adjust the pH of the solution to 4.6 with hydrochloric acid, transfer and dilute the pH-adjusted solution to a 50mL centrifuge tube, and let it stand for 30min;
[0045] Centrifuge the above solution at 4℃, 5000rpm for 15min, take the supernatant and add BaCl 2 Make the concentration to 0.05M, after mixing, get about 25mL containing BaCl 2 的 CPP raw material solution;
[0046] Contains BaCl in the above 2 Add about 50mL of absolute ethanol to the CPP raw material solution, mix well, and let stand overnight at 4°C;
[0047] Centrifuge the solution after standing at 4°C and 5000rpm for 15min, discard the supernatant, and add about 10mL of 0.125M H in the precipitate. 2 SO 4 , Then filter with filter paper;
[0048] Take the...
Embodiment 2
[0058] This example provides a method for detecting casein phosphopeptides in dairy products, which uses the purified CPP raw material prepared in Example 1 as a standard for detection, and the obtained standard curve 2 (such as figure 2 The linear correlation coefficient shown in) is 0.998, and the linear range is 0.1-20mg / 100mL; the detection steps of the casein phosphopeptide product of this example are basically the same as those in Example 1, except that 20.5370g of liquid milk samples are weighed. Detection, the chromatogram obtained is as Image 6 As shown, the peak area of the CPP sample is 98, and the CPP content in the liquid milk is calculated as 0.12 mg / 100 g by using the standard curve 2 and formula 1.
Embodiment 3
[0060] This example provides a method for detecting casein phosphopeptides in dairy products, which uses the purified CPP raw material prepared in Example 1 as a standard for detection, and the obtained standard curve 3 (such as image 3 The linear correlation coefficient shown) is 0.999, and the linear range is 0.1-20mg / 100mL; the detection steps of the casein phosphopeptide product in this example are basically the same as those in Example 1, except that 5.3450g milk powder sample is weighed for detection , The resulting chromatogram is as Figure 7 As shown, the peak area of the CPP sample is 1300, and the content of CPP in the milk powder is 148.0mg / 100g calculated by using the standard curve 3 and formula 1.
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