Expression, purification and application of E.coli O157:H7 flagellin H7 antigen segment

A flagellin and protein technology, which is applied in the field of genetic engineering technology and detection reagents, can solve the problems of long experiment time and unsuitability for rapid detection, and achieve the effects of easy purification, avoiding interference, good antigenicity and specificity
CN103804474AActive Publication Date: 2014-05-21中国人民解放军东部战区疾病预防控制中心
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
中国人民解放军东部战区疾病预防控制中心
Publication Date
2014-05-21

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Abstract

The invention discloses expression, purification and application of (i) E.coli ( / i) O157:H7 flagellin H7 antigen segment, relating to the field of genetic engineering technology, vaccine and diagnostic reagent. According to computer analysis, strong antigen epitope in (i) E.coli ( / i) O157:H7Flic protein is screened out, totally 131 amino acid, from 215th amino acid to 345th amino acid, codon preferred by prokaryote is selected for chemical synthesis of a brand-new gene sequence of the antigen epitope, by genetic engineering technology, the gene segment is expressed, and the strong antigen epitope segment of the (i) E.coli ( / i) O157:H7Flic protein is prepared. The expressed protein can be used for detection of (i) E.coli ( / i) O157:H7 infected antibody and preparation of monoclonal antibody and polyclonal antibody.
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Description

Technical field

[0001] The present invention involves E. Coli O157: The expression, purification and application of H7 whipped protein H7 antigen fragment, through chemical synthesis E. Coli O157: H7 whipped protein H7 antigen new gene fragment, using genetic engineering technology to prepare recombinant protein.Through computer analysis, the whipped protein H7 antigen fragment containing a strong antigen surface is screened, and a codon that prefers the primary biological preference to chemically synthesize a new gene sequence. Use genetic engineering technology to express E. Coli O157: The detection of H7 antibodies and the preparation of monoclonal antibodies, which are the fields of genetic engineering technology and detection reagents. Background technique

[0002] E. Coli O157: H7 is a pathogenicity E. Coli One of the serum types, the infectious diarrhea caused by it is the extremely serious intestinal infectious disease found in recent years.In addition to causing diarrhe...

Claims

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