An antibiotic-free quadrivalent disease-pest-resistant and herbicide-resistant plant expression vector and its application

Abstract

The invention belongs to the technical field of biology and particularly relates to an antibiotic-free tetravalent disease and pest-resisting and herbicide-resisting plant expression vector and an application thereof. A T-DNA (Deoxyribonucleic Acid) region structure of the vector is as follows: right margin-35S promoter-positive bivalent disease-resisting sequence-intron-negative bivalent disease-resisting sequence-OCS terminator-Ubi promoter-insect-resisting gene-NOS terminator-NOS promoter-herbicide-resisting gene-NOS terminator-left margin; an antibiotic gene does not exist in left and right margins of a T-DNA region; the positive bivalent disease-resisting sequence is a sugarcane floral leaf virus and sugarcane yellow leaf virus bivalent interference sequence; the DNA sequence is shown as SEQ ID NO.1. The obtained plant expression vector can be used for transforming to obtain four transgenic crops with resistance; the obtained transgenic crops have no antibiotic resisting genes and the safety of the transgenic crops can be improved. The four transgenic crops with the resistance can be obtained by the same time of transformation; compared with single-resistance genetic transformation, the efficiency can be improved by more than three times.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an antibiotic-free quadrivalent pest-resistant and herbicide-resistant plant expression vector and its application. Background technique [0002] Sugarcane yellow leaf disease and mosaic disease are viral diseases that seriously endanger the agricultural production of sugarcane and the healthy development of the sugar industry. Sugarcane yellow leaf disease belongs to the family Flavivirus ( Luteoviridae ) potato leafroll virus ( Polerovirus ), the virus is composed of a protein shell and a single-stranded, positive-sense RNA (ssRNA) wrapped in it. The content in sugarcane is very low, and it is only distributed in the cytoplasm of its phloem sieve tube companion cells. It has occurred in more than 30 sugarcane-growing countries and regions in the world. Since Wang Bohui reported the discovery of sugarcane yellow leaf disease in Guangxi in 2003, it has also appeared in ...

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Problems solved by technology

However, so far, transgenic sugarcane resistant to two viral diseases and resistant to insects and herbicides has not been reported

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