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Extraction method of thuricin

An extraction method and technology of extraction method, applied in the field of separation and purification of natural substances, can solve the problems of separation conditions and environmental requirements, low concentration and biological activity, extraction technology limitations, etc., and achieve the effect of easy operation, simple steps, and cost reduction

Inactive Publication Date: 2014-05-21
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the rapid development of biotechnology, the application of conventional extraction technology in these fields is limited, because most of the raw liquid of biological products has low concentration and biological activity, and has strict requirements on separation conditions and environment

Method used

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Examples

Experimental program
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Embodiment 1

[0017] The bacterial powder was diluted with sterilized beef extract peptone liquid medium, then transferred to slant medium, cultivated in an incubator at 30°C for 2 days, and then placed in a 4°C refrigerator for refrigeration. The refrigerated strains were inserted into the seed medium and cultured at 30°C until the end of logarithmic growth. Use a 500ml Erlenmeyer flask to pack 20ml of the culture solution, seal it with kraft paper, insert the seeds of the liquid medium after sterilization, and then place it on a shaker at 30±1°C with a rotation speed of 180r / min for 48h. The fermented liquid was placed in a high-speed refrigerated centrifuge, centrifuged at 8820 g for 15 min, and the supernatant was recovered. Put 15g of the fermentation supernatant into a 50ml Erlenmeyer flask, and then add 3.15g of PEG (8000) and 2.85g of dipotassium hydrogen phosphate into it. Place on a shaker and shake at 200r / min for 1h. Put the drug-added liquid in a high-speed refrigerated centr...

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PUM

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Abstract

The invention relates to an extraction method of thuricin. The extraction method comprises the following process routes of: activation of thuricin bacillus; cultivation; two aqueous phase extraction to obtain liquor containing thuricin. The extraction method provided by the invention is a production process of obtaining thuricin, which is simple and convenient to operate, low in production cost, free from environmental pollution in the extraction process and free from organic solvent residues.

Description

technical field [0001] The invention relates to the field of separation and purification of natural substances, in particular to a process method for separating and extracting thurokinin from Bacillus thuringiensis strains by using a two-phase method. Background technique [0002] Thuringin, also known as β-exotoxin, is a nucleoside analogue produced by certain strains of Bacillus thuringiensis. It is resistant to high temperature, easily soluble in water, and stable in nature. Surgeonin competes with ATP for the binding site of the enzyme, causing the death of pests. It has varying degrees of toxicity to harmful insects such as Lepidoptera, Diptera, and Orthoptera, as well as nematodes and mites. Recent studies have found that the toxicity of thuringienne is lower than that of general chemical pesticides, especially after it is proved that it is safe for mammals, the research and application of β-exotoxin has attracted more and more attention. [0003] At present, there ar...

Claims

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Application Information

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IPC IPC(8): C12P19/40C07H19/167C07H1/06C12R1/07
Inventor 杨玉焕
Owner NORTHEAST FORESTRY UNIVERSITY
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