Endophytic fungi of Sargassum chinensis in Fujian and Zhejiang and methods and applications thereof for producing huperzine A

An endophytic fungus and huperzine A technology, applied in the field of microorganisms, can solve the problems of inability to apply industrialized production, low huperzine A yield, etc., and achieve the effect of solving the bottleneck problem

Active Publication Date: 2016-08-17
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the currently reported strains of huperzine A have low yields and cannot be applied to industrial production.

Method used

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  • Endophytic fungi of Sargassum chinensis in Fujian and Zhejiang and methods and applications thereof for producing huperzine A
  • Endophytic fungi of Sargassum chinensis in Fujian and Zhejiang and methods and applications thereof for producing huperzine A
  • Endophytic fungi of Sargassum chinensis in Fujian and Zhejiang and methods and applications thereof for producing huperzine A

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Experimental program
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Embodiment 1

[0035] 1. Observation of bacterial strain micromorphology: Two kinds of endophytic fungi that produce huperzine A of the present invention are spot-inoculated in the center of the flat plate, and then the sterilized cover glass is inserted obliquely at 45 ° in the flat plate of the inoculated bacteria (2 Slices / plates) were cultivated in a fungal incubator at 28°C. After the strains had grown to a certain extent (6 days), the inserts (in an ultra-clean bench) were taken and observed under a microscope.

[0036] see figure 1 It is the micromorphological figure of the cereus tearing fungus MY183 of the present invention, and the microscopic shape of the tearing cereus spore MY183 is: hyphae without septa, branched, smooth, tubular, with unequal spacing, spores oval, single spore.

[0037] see figure 2 It is the micromorphological figure of the covered carbon group bacteria MY311 of the present invention, and the microscopic morphology of the covered carbon group bacteria MY311 ...

Embodiment 2

[0054] One, the collection of endophytic fungus of Sargassum pine in Fujian and Zhejiang provinces of the present invention

[0055] 1. Separation of endophytic fungi from Sargassum lanceolata collected from Guanzhai Mountain, Liancheng County, Longyan City, Fujian Province according to the separation method of Shi Wei et al. Rinse the collected fresh Chinese fir from Fujian and Zhejiang with tap water, immerse in 75% ethanol (5min), and rinse with sterile water for 5 times; dry it with sterile filter paper and then immerse it in 0.1% mercuric chloride (about 1min). ), rinse again with sterile water, blot dry with sterile filter paper, and finally soak in 75% ethanol for 30 seconds, and blot dry with sterile filter paper. Different plant tissue materials (ie roots, stems, leaves) were divided into approximately 5 mm long size with sterilized scissors. Each sample was then transferred to PDA plates containing 3% streptomycin for the isolation of endophytic fungi. Cultivate fo...

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Abstract

The invention relates to the technical field of microorganisms, in particular to Fujian and Zhejiang phlegmariurus endophytic fungi, as well as a method and application of the Fujian and Zhejiang phlegmariurus endophytic fungi for producing huperzine-A. One Fujian and Zhejiang phlegmariurus endophytic fungus provided by the invention is named ceriporia lacerata MY183, and is preserved in CCTCC (China Center for Type Culture Collection) with the preservation number CCTCC No. M2013644. According to the other technical scheme, the other Fujian and Zhejiang phlegmariurus endophytic fungus provided by the invention is named hypoxylon sp. MY311, and is preserved in CCTCC (China Center for Type Culture Collection) with the preservation number of CCTCC No. M2013645. The endophytic fungi for producing the huperzine-A is extracted from Fujian and Zhejiang phlegmariurus; the fact that the huperzine-A can be produced by the ceriporia lacerata and the hypoxylon is discovered for the first time. The Fujian and Zhejiang phlegmariurus endophytic fungi are an important microorganism for searching for new sources of the huperzine-A, and have relatively-high application value.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to an endophytic fungus of Sargassum chinensis from Fujian and Zhejiang provinces and a method and application thereof for producing huperzine A. Background technique [0002] Alzheimer's disease is a serious, degenerative brain disease. Due to its high incidence and high disability rate, it has caused serious harm and huge economic burden to society and families. With the aging of society, the incidence of Alzheimer's disease According to statistics, there are more than 50 million elderly people in the world suffering from different degrees of Alzheimer's disease, so the treatment of Alzheimer's disease is a major problem in the current society. [0003] Huperzine A [(-) Huperzine A, Hup A] is a new effective monomer of lycopodium alkaloids isolated from Huperzine serrata in 1986 by Chinese scientist Liu Jiasen. Pharmacological experiments show that it can inhibit the activity of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P17/12C12R1/645
Inventor 吴水生张方方郑雅媗刘海元
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
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