Method for identifying whether organic matters are utilized by ANAMMOX (anaerobic ammonium oxidation) bacteria in ANAMMOX reactor sludge

Abstract

The invention discloses a method for identifying whether organic matters are utilized by ANAMMOX (anaerobic ammonium oxidation) bacteria in ANAMMOX reactor sludge, relating to a method for identifying whether organic matters are utilized by microorganisms in ANAMMOX reactor sludge. The invention provides the method for identifying whether organic matters are utilized by ANAMMOX bacteria in ANAMMOX reactor sludge without enriching high-purity ANAMMOX bacteria, separating, identifying, purifying and purely culturing. The method comprises the step of firstly, marking ammonia nitrogen in an ANAMMOX reactor by using 15N, marking the organic matters in the ANAMMOX reactor by using 13C, and then, carrying out ANAMMOX reaction; secondly, observing by using a TEM (Transmission Electron Microscope); thirdly, preparing a conductive sample; and fourthly, obtaining a result according to a NanoSIMS drawing. The method is applied to the fields of water treatment and microorganisms.

Description

technical field [0001] The invention relates to a method for identifying whether microorganisms in ANAMMOX reactor sludge utilize organic matters. Background technique [0002] With the development of global industrialization and urbanization, the rapid growth of population and the great increase of water consumption, the increasing shortage of water resources is threatening the survival and development of human beings. my country is one of the countries with severe water shortages. The amount of fresh water only accounts for 1 / 4 of the world average, and it has entered the early stage of water resource crisis. At the same time, the imbalance in the geographical distribution of water sources has made the contradiction of fresh water shortage more prominent. The more serious problem is that my country's water resources are seriously polluted. From general pollutants to toxic and harmful pollutants, the coexistence of point sources and non-point sources has formed, domestic po...

AI Technical Summary

Problems solved by technology

Although the use of isotope tracer technology has proved that some specific strains of known ANAMMOX bacteria can utilize organic matter, but relying on isolation and identification methods cannot meet the needs of research on ANAMMOX bacteria in different ANAMMOX sludge

[0008] Another researcher studies the physiology and biochemistry of ANAMMOX bacteria in ANAMMOX sludge through enrichment methods, but the enriched microorganisms also contain non-ANAMMOX bacteria, so the experimental results will be interfered by non-ANAMMOX bacteria, and the enrichment The process may also lead to changes in the microbial population, affecting the accuracy of the results

[0009] Since ANAMMOX bacteria have not been purely cultured, the research on the utilization of organic matter by ANAMMOX bacteria is based on the reactor level. As a mixed culture system, it can only prove that organic matter has no effect on the ANAMMOX process, and the results are all determined by the concentration of substances in the water. It is obtained indirectly from the change analysis, which cannot provide direct evidence for the utilization of organic matter by ANAMMOX bacteria; and it is impossible to know which microorganism or microorganisms in the enrichment are utilizing organic matter

Even if the ANAMMOX bacteria in the sludge are enriched and cultured and the existing isotope tracer technology is used, the experimental results still lack credibility and convincing because the pure culture cannot be achieved and the interfering factors in the microorganisms cannot be ruled out.

Images