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Camellia oleifera Abel saponin compound, its preparation method, application and antitumor drug prepared from the same

An anti-tumor drug, the technology of camellia oleifera saponin, which is applied in the field of anti-tumor drugs and can solve problems such as different combination methods and complex aglycon structures

Inactive Publication Date: 2014-06-04
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Camellia oleifera saponins are mostly oleanane-type pentacyclic triterpenoid saponins, including aglycone, sugar, and organic acid. However, the complex structure of aglycone, many types of sugar parts and different combinations have resulted in a large number of saponins in camellia oleifera. , which poses a challenge to the further purification and separation of camellia oleifera saponins
Therefore, although studies have found that the total saponin extract of camellia oleifera has anti-tumor activity, it is still unknown which monomer saponin plays the role, and in-depth research is needed

Method used

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  • Camellia oleifera Abel saponin compound, its preparation method, application and antitumor drug prepared from the same
  • Camellia oleifera Abel saponin compound, its preparation method, application and antitumor drug prepared from the same
  • Camellia oleifera Abel saponin compound, its preparation method, application and antitumor drug prepared from the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0082] Embodiment 1 has the preparation of the camellia saponin compound of structure shown in formula I

[0083] extract:

[0084] Take 2000g of dry Camellia oleifera root (collected from Qichun, Hubei in November 2011), crush it into sawdust with a grinder, add 20.0L of 70% ethanol aqueous solution, soak at 40°C for 10h, heat to reflux, and extract 3.0h, collect the extract, filter the extract, concentrate under reduced pressure to obtain 602g of liquid extract.

[0085] Macroporous resin enrichment:

[0086] Take the liquid extract obtained in the extraction step, add 6.02L of water, stir to dissolve, filter with a 100-mesh filter cloth, and centrifuge the filtrate at 12000r / min for 10min. After centrifugation, 5.3L of supernatant is obtained. Pore ​​resin was further separated, and eluted successively with 10L water, 15L 30% ethanol aqueous solution, and 15L 70% ethanol aqueous solution, collected 70% ethanol aqueous solution eluted components, and concentrated under red...

Embodiment 2

[0112] Embodiment 2 has the preparation of the camellia saponin compound of structure shown in formula I

[0113] extract:

[0114] Take 2000g of dry Camellia oleifera root (collected from Qichun, Hubei in November 2011), crush it into sawdust with a grinder, add 40.0L of 30% ethanol aqueous solution, soak at 30°C for 12h, heat to reflux, and extract three times , 0.5h each time, collect the extract, filter the extract, concentrate under reduced pressure, and obtain 628g of liquid extract.

[0115] Macroporous resin enrichment:

[0116] Take the liquid extract obtained in the extraction step, add 0.628L of water, stir to dissolve, filter with a 300-mesh filter cloth, and centrifuge the filtrate at 12000r / min for 10min. After centrifugation, 5.8L of supernatant is obtained. Pore ​​resin was further separated, and eluted with 10L water, 15L 60% ethanol aqueous solution, 10L 95% ethanol aqueous solution successively, collected 95% ethanol aqueous solution elution component, con...

Embodiment 3

[0127] Embodiment 3 has the preparation of the camellia saponin compound of structure shown in formula I

[0128] extract:

[0129] Take 2000g of dry Camellia oleifera root (collected from Qichun, Hubei), grind it into sawdust with a grinder, add 10.0L of 95% ethanol aqueous solution, soak at 50°C for 6h, heat to reflux, and extract twice, each After 1 hour, the extract was collected, filtered, and concentrated under reduced pressure to obtain 638 g of liquid extract.

[0130] Macroporous resin enrichment:

[0131] Take the liquid extract obtained in the extraction step, add 6.0L of water, stir to dissolve, filter with a 200-mesh filter cloth, and centrifuge the filtrate at 12000r / min for 10min. After centrifugation, 5.5L of supernatant is obtained. Porous resin was further separated, and eluted with 10.0L water, 15.0L40% ethanol aqueous solution, and 15.0L L80% ethanol aqueous solution successively, collected 80% ethanol aqueous solution eluted fraction, concentrated under ...

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Abstract

Belonging to the technical field of pharmaceutical chemistry, the invention discloses a Camellia oleifera Abel saponin compound, its preparation method, application and an antitumor drug prepared from the same. The Camellia oleifera Abel saponin compound has a structure shown as formula I, and has certain inhibiting effect on liver cancer cells, breast cancer cells, melanoma cells and lung cancer cells, also can effectively inhibit the liver tumor growth. Thus, the Camellia oleifera Abel saponin compound has significant antitumor activity, and can be used for preparing anti-tumor drugs.

Description

technical field [0001] The invention belongs to the technical field of medicinal chemistry, and in particular relates to a camellia oleifera saponin compound, its preparation method, application and antitumor drug prepared therefrom. Background technique [0002] Camellia oleifera (Camellia oleifera Abel), also known as tea seed tree, camellia oil tree, white flower tea, is an evergreen small tree of the family Theaceae (Theaceae) Camellia Linn, mainly distributed in tropical and subtropical regions. In my country, camellia oleifera is produced in the southwest and southeast regions, covering 17 provinces. Camellia oleifera is an important woody edible oil tree species, and it is called "the world's four largest woody oil tree species" together with olive, oil palm and coconut. Camellia oleifera oil is obtained by pressing oil from the seeds of camellia oleifera. The unsaturated fatty acid content of tea oil is as high as 90%, which is much higher than that of rapeseed oil,...

Claims

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Application Information

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IPC IPC(8): C07J63/00C07H15/256C07H1/08A61K31/704A61P35/00
Inventor 许琼明杨世林李夏李笑然刘艳丽陈重
Owner SUZHOU UNIV
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