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Imaging System for Blood Cells in Flow Cytometry

A flow cytometer and imaging system technology, which is applied to the field of blood cell imaging systems in flow cytometers, can solve the problems of difficulty in providing quantification and statistical data of cell populations, limited number of cells, etc., and achieves convenient use, low cost, Aesthetically pleasing effect

Inactive Publication Date: 2016-09-14
JIANGXI SCI & TECH NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

To obtain images of cells, researchers must use a microscope to observe, but the number of cells that can be observed with a microscope is very limited, and it is difficult to provide quantitative and statistical data on cell populations
Therefore, using traditional cell analysis techniques, we can only face such a dilemma. Currently, there is no technique that can provide both statistical data of cell populations and obtain cell images.

Method used

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  • Imaging System for Blood Cells in Flow Cytometry
  • Imaging System for Blood Cells in Flow Cytometry

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Embodiment

[0017] Example: see figure 1 As shown, the imaging system for blood cells in the flow cytometer of the present invention includes a laser radiation and beam shaping unit, a flow detection cell, and an imaging unit for side scattered light and fluorescence. The first laser 110 and the second laser 120 arranged vertically in the laser radiation unit pass through the half-transparent mirror 130 at an angle of 45 degrees to the optical axis, and pass through the first cylindrical lens 140 and the second After cylindrical lens 150 and single lens 160 are reshaped, an elliptical light spot is formed to focus on the sample in the flow detection cell 200 in front, thereby generating scattered light and fluorescence. 150 and the single lens 160 are on the same axis; in the direction of 270 degree angle with the beam irradiation direction of the laser 110, the side scattered light and the excited fluorescence are collected by the objective lens 320 of large numerical aperture after bein...

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Abstract

The invention discloses an imaging system for blood cells in a flow cytometry. The imaging system comprises a laser device, a semi-transparent and semi-reflection mirror, a cylindrical lens, a single lens, a flowing detection tank, a collecting lens, an objective lens, a single lens, a collimating lens and a holographic narrow band pass filter. The imaging system has the following beneficial effects that numerical values of a scattered light signal and a fluorescence signal of each cell can be obtained so that the statistical data of a cell group can be obtained; the blood cells of the flow cytometry can be imaged to realize research on a blood cell image.

Description

technical field [0001] The invention relates to the technical field of cell imaging, in particular to an imaging system for blood cells in a flow cytometer. Background technique [0002] Flow cytometer is an advanced scientific and technical equipment integrating photons, electronics, fluid mechanics, cytochemistry, biology, immunology, laser and computer and other disciplines. It is widely used in clinical medicine, cytology, Biology, microbiology, pharmacy, reproductive science and other fields. It is one of the advanced instruments in modern scientific research, known as the "CT" of the laboratory. It performs one-by-one, multi-parameter, rapid qualitative and quantitative analysis or sorting technology on single cells or biological particles in a fast linear flow state. It has fast detection speed, multiple measurement parameters, large collection data, comprehensive analysis, and sorting purity. High, flexible methods and other characteristics play an important role i...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N15/14
Inventor 刘国栋徐浩郝希应董健
Owner JIANGXI SCI & TECH NORMAL UNIV