Soluble powder of Sargassum polysaccharide for prevention and treatment of colibacillosis in chickens
A technology of sargassum polysaccharides and colibacillosis, which is applied in the direction of antibacterial drugs and powder delivery, and can solve problems such as food safety and unsatisfactory immune effects of bacteria vaccines
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Embodiment 1
[0010] Preparation of Sargassum polysaccharide
[0011] Weigh the Sargassum powder, add 20 times of distilled water and mix well, soak overnight, heat in 80°C water bath for 8h, extract the solution by centrifuging at 3000r / min for 10min, concentrate the obtained supernatant to 1 / 4 volume with a vacuum rotary evaporator, add 3 Double the volume of 95% ethanol, precipitate overnight, centrifuge at 3000r / min for 10min, discard the supernatant, and vacuum freeze-dry the precipitate to obtain Sargassum polysaccharide.
[0012] Preparation of sargassum polysaccharide soluble powder
[0013] Make the sargassum polysaccharide prepared in step into a solution with a mass concentration of 5% with distilled water, put it in a boiling water bath for 20 minutes; take it out and cool it, centrifuge it at 2000r / min for 10 minutes, and collect the supernatant; according to the mass ratio of 1:20 Add distilled water to the residue after centrifugation, continue to bathe in a boiling water...
Embodiment 2
[0015] Basically the same as in Example 1, except that in step , heat in a water bath at 70°C for 9 hours.
Embodiment 3
[0017] Basically the same as Example 1, only step was heated in a water bath at 90°C for 6 hours.
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