A preparation method of upconversion luminescent nanoprobe and its application in the determination of the antioxidant activity of substances
A technology of anti-oxidation activity and nano-probes, applied in luminescent materials, chemical instruments and methods, fluorescence/phosphorescence, etc., to achieve the effect of easy realization, easy promotion, and simple operation
Inactive Publication Date: 2015-09-16
HEFEI UNIV OF TECH
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At present, the work of evaluating the antioxidant activity of drugs based on this upconversion luminescence quenching-recovery mode has not been reported
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[0037] The present invention will be further described below in conjunction with accompanying drawing and specific embodiment of the present invention:
[0038] 1. Preparation of upconversion luminescent nanoparticles:
[0039] 207.4mg Y(CH 3 CO 2 ) 3 ,70mg Yb(CH 3 CO 2 ) 3 and 6.9mg Er(CH 3 CO 2 ) 3 Disperse in a mixed solution of 6mL oleic acid and 14mL octadecene, and react under vacuum at 150°C for 1 hour to obtain a precursor solution of rare earth ions;
[0040] 100mg NaOH and 148mg NH 4 After F is mixed, it is ultrasonically dispersed in 10 mL of methanol, and then added dropwise to the precursor solution of the rare earth ion. After stirring and reacting for 30 minutes, heat to 80°C and vacuumize for 10 minutes, and then heat up to 300°C to react one under the protection of nitrogen. hours, cooled to room temperature, added 20-40 mL of acetone, centrifuged, removed the supernatant, washed the precipitate with ethanol 3 times and dried in vacuum to obtain Yb a...
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Abstract
The invention discloses a preparation method for an up-conversion luminescent nano-probe. The preparation method comprises the following steps of performing amination surface modification on up-conversion luminescent nanoparticles of Yb and Er-doped NaYF4, NaGdF4 or CaF2 by using lysine, polyethyleneimine or polyacrylamide, and performing amidation surface coupling by using carboxyl of carminic acid. The prepared probe can be used for measuring the antioxidant activity of a medicament by measuring changes in luminescence intensity before and after the addition of a mixed solution of the medicament to be measured and free hydroxyl into an aqueous solution of the up-conversion luminescent nano-probe. The measurement method has the advantages of simplicity and convenience in operation, sensitivity, high speed, effectiveness and resistance to the interference of self-activating signals, and can be used for rapidly screening medicaments with high antioxidant activities and rapidly comparing the antioxidant activities of different medicaments, thereby providing effective technical support for the research and development of antioxidant medicaments; the technical problems of a conventional method are solved, and a novel detection method is provided for the evaluation of the medicament.
Description
technical field [0001] The invention belongs to the technical field of up-conversion luminescence materials and their applications, and in particular relates to an up-conversion luminescence nanoprobe and its application in measuring the antioxidant activity of substances. Background technique [0002] Lanthanide-doped up-conversion luminescent nanomaterials are excited by low-energy near-infrared photons to emit high-energy visible light, which has many significant advantages, such as large anti-Stokes shift and small autofluorescence interference of the sample itself. Therefore, it has a wide range of applications in the fields of biosensing, bioimaging and photodynamic therapy. For example, Liu X.G. et al. reported in the journal "J.Am.Chem.Soc." that they used manganese dioxide nanosheets to quench the luminescence of upconversion luminescent nanoparticles, and then added glutathione to restore the upconversion luminescence nanoparticles. The luminescence of glutathione...
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IPC IPC(8): C09K11/02C09K11/85C09K11/61G01N21/64
Inventor 梅青松李炳南张勇
Owner HEFEI UNIV OF TECH