Method utilizing cyclodextrin to assist plant sterol composition to prepare androstane-4-ene-3,17-diketone

A technology for phytosterol and composition, applied in the field of preparing androst-4-ene-3, can solve the problems of low solubility, increase of miscellaneous bacteria, excessive fermentation of microorganisms and the like

Inactive Publication Date: 2014-06-18
TIANJIN JINYAO GRP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yang Ying pointed out in the article (Research progress of microbial degradation of sterol side chain and conversion of androst-4-ene-3??17-dione, Microbiology Bulletin, 2006 33 (6), 142-145) sterol substrates in The problem of poor solubility and serious product (substrate) inhibition in aqueous media has plagued the entire steroid industry. Sterols are hydrophobic compounds with extremely low solubility in water, which leads to incompatible substrates and microbial cells. Good contact, lower conversion rate and longer conversion time
[0006] Utilize mycobacterium to carry out fermentation at the same time, the time is longer, often up to 72 hours,

Method used

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  • Method utilizing cyclodextrin to assist plant sterol composition to prepare androstane-4-ene-3,17-diketone
  • Method utilizing cyclodextrin to assist plant sterol composition to prepare androstane-4-ene-3,17-diketone
  • Method utilizing cyclodextrin to assist plant sterol composition to prepare androstane-4-ene-3,17-diketone

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-1-a

[0061] Species: Mycobacterium: Mycobacterium fortuitum. NRRL B-8153.

[0062] Take 10 different batches of corn steep liquor and ferment them respectively according to the quantity and process in this example. Except corn steep liquor, other materials are the same batch of materials.

[0063] Seed culture stage:

[0064] Seed medium (per liter):

[0065] 40ml corn syrup

[0066] 5.2g NaNO 3 ,

[0067] 0.8g NH 4 h 2 PO 4

[0068] 5.3g glucose

[0069] The rest is sterile water

[0070] Medium volume: 200ml / flask

[0071] Inoculum volume: Inoculate 3ml of cell suspension from agar slant per 200ml of new medium

[0072] Container: 1000ml conical flask

[0073] Stirring: Shaker (1”throw) 200rpm

[0074] Temperature: 30°C

[0075] Growth time: 72 hours culture

[0076] Note: A good culture organism is indicated if a yellow cell pellet forms at the bottom

[0077] method:

[0078] Inoculum for biotransformation was prepared in culture medium. Inoculate the Erlenmeye...

Embodiment 1-1-b

[0099] The material and method of this example are basically the same as those of Example 1-1-a, except that 0.01 mole of γ-cyclodextrin is added to the sunflower oil in which the phytosterol composition (0.05 mole) is dispersed. See the table below for the fermentation time of steroidal fermentation culture.

[0100]

Embodiment 1-2-b

[0102] The materials and methods of this example and Example 1-1-b are basically the same, the difference is that the amount of emulsifier sunflower oil is 50ml, and the type, molar amount and results of cyclodextrin are shown in the table below.

[0103]

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Abstract

The invention relates to a method utilizing cyclodextrin to assist a plant sterol composition to prepare androstane-4-ene-3,17-diketone, and provides a preparation method utilizing nocardia or mycobacterium to ferment a plant sterol composition so as to obtain androstane-4-ene-3,17-diketone and/or androstane-1,4-diene-3,17-diketone, wherein cyclodextrin is added during the fermentation process, and the mole ratio of the cyclodextrin to the plant sterol composition is 1:10 to 1:2.

Description

field of invention [0001] The present invention generally relates to a process for the preparation of androst-4-ene-3,17-dione, which effectively improves the production of androst-4-ene with a phytosterol composition as a substrate by adding a small amount of cyclodextrin The yield of -3,17-diketone shortens the fermentation time, which is very beneficial to industrialization. Background technique: [0002] Androst-4-ene-3, 17-dione (androst-4-ene-3, 17-dione, referred to as androstenedione or AD) is an irreplaceable intermediate of steroid hormone drugs, which plays an important role in the body. very important regulation. It can be said that almost all steroid hormone drugs are produced with AD as the starting material. Such as for the production of sex hormones, progesterone, anabolic hormones and corticosteroids, but also for the synthesis of more than 100 kinds of drugs such as hydrocortisone, prednisone oxide, progesterone, estrenol, dexamethasone, etc. It is an es...

Claims

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Application Information

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IPC IPC(8): C12P33/00C12R1/32C12R1/365
Inventor 孙亮赵琳
Owner TIANJIN JINYAO GRP
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