Method of separating histidase enantiomer by chitosan-modified gold nanochannel film and detecting method thereof

A gold nanochannel and nanochannel technology, which is applied in the field of separation and detection of histidine enantiomers based on nanochannels combined with surface-enhanced Raman scattering spectroscopy, can solve the problems of difficult portability of instruments, complex detection, and large sample volume, and achieve The effects of shortened detection time, simple separation process, and broad application prospects

Inactive Publication Date: 2014-07-02
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are complex and time-consuming, the instruments are not easy to carry, and require a large amount of samples, which are limited in continuous monitoring and on-site determination

Method used

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  • Method of separating histidase enantiomer by chitosan-modified gold nanochannel film and detecting method thereof
  • Method of separating histidase enantiomer by chitosan-modified gold nanochannel film and detecting method thereof
  • Method of separating histidase enantiomer by chitosan-modified gold nanochannel film and detecting method thereof

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Embodiment

[0027] 1 Preparation of gold nanochannel membrane

[0028] Gold was deposited on a 50nm porous polycarbonate (PC) film and a 100nm porous anodized aluminum oxide film (AAO) by electroless deposition. The PC membrane was immersed in anhydrous methanol for 30min to wash away the impurities adsorbed on the base membrane, and the AAO membrane was soaked in 30% hydrogen peroxide for 24h. Then put the cleaned PC membrane and AAO membrane into 0.026mol / L SnCl 2 and 0.007mol / L CF 3 COOH50% methanol / water solution, placed in a shaker and shaken for 45min at a speed of 90r / min to make the Sn 2+ Evenly adsorbed on the surface of the basement membrane and membrane pores, the sensitized membrane was taken out and rinsed with methanol for 3 times, and then the membrane was put into freshly prepared 0.029mol / L Ag(NH 3 ) 2 + In the solution for 15 minutes, and continue to pass nitrogen, so that the surface of the membrane is fully activated. After taking it out, wash it 3 times with met...

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Abstract

The invention discloses a method of separating histidase enantiomer by a chitosan-modified gold nanochannel film. The method comprises the following steps: by taking a polycarbonate film and an aluminum oxide film as base films, adopting a chemical deposition method to prepare a gold nanochannel film; self-assembling chitosan onto the pore wall of the gold nanochannel to form a functional nanochannel film with chiral site selectivity on the surface; and separating D-histidine, L- histidine in a chiral manner by utilizing excellent separation ability of the nanochannel. During detection, silver sol is used as a surface enhanced Raman substrate to enhance SERS (surface enhanced raman scattering) effect of D-histidine, L-histidine, so that selectivity and sensitivity of the substance are improved to detect the D-histidine and the L-histidine at the same time. The invention provides a quicker and more convenient method for separating and detecting the chiral substance by constructing a coupling device of a nanochannel separating tank and an SERS detecting system, and shows unique advantages and wide application prospect of the method.

Description

technical field [0001] The invention relates to the field of materials and sensing technology, and is a molecular-level membrane separation detection technology based on nanochannel membrane materials, specifically a method for separating and detecting histidine enantiomers based on nanochannels combined with surface-enhanced Raman scattering spectroscopy . Background technique [0002] Since Richard Feynman, a famous physicist and Nobel laureate, proposed the concept of nanotechnology in 1959, nanotechnology has experienced more than 50 years of development and has become a modern science (quantum mechanics, mesoscopic physics, molecular biology, etc.) and The product of cross-combination of modern technology (microelectronics, computer technology, scanning tunneling microscope technology, nuclear analysis technology, etc.), is the frontier of modern scientific research. [0003] In recent years, nanochannel technology, as an important branch in the field of nanobiotechnol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D233/64G01N21/65
CPCC07D233/64G01N21/658
Inventor 黄杉生柳悦谢利马腾飞杨乐乐
Owner SHANGHAI NORMAL UNIVERSITY
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