Supercharge Your Innovation With Domain-Expert AI Agents!

Method and device for extracting chlorella oil by virtue of wet method

A technology of chlorella and oil, which is applied in the direction of fat oil/fat production and fat production, can solve the problems of low organic solvent extraction rate and large solvent loss, and achieve the effects of saving heat, increasing extraction rate, and reducing the amount of addition

Inactive Publication Date: 2014-07-02
CHINESE ACAD OF AGRI MECHANIZATION SCI
View PDF5 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to propose a method and device for wet extraction of chlorella oil, to solve the problems of low organic solvent extraction rate and large solvent loss in the process of wet extraction of chlorella oil

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method and device for extracting chlorella oil by virtue of wet method
  • Method and device for extracting chlorella oil by virtue of wet method
  • Method and device for extracting chlorella oil by virtue of wet method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Put 100g (about 100mL) of chlorella mud 1 with a water content of 80% obtained by flocculation and concentration into a packaging bag, add 0.2mL of concentrated sulfuric acid, 0.05g of sodium carbonate, 50mL of chloroform, and 100mL of methanol, exhaust, and seal. Put the packaging bag into the container 3, increase the pressure to 600MPa, and keep the pressure for 600S. After taking it out, put it into a centrifuge tube, and centrifuge at 4500r / min for 18min. Pour the supernatant into a separatory funnel, add 50 mL of chloroform, shake well, and let stand for 3 h. The lower aqueous layer was discarded, and the supernatant was subjected to reduced-pressure rotary evaporation, and the chlorella oil obtained by wet extraction was obtained after the concentration was completed.

Embodiment 2

[0066] Put 100g (about 100mL) of chlorella mud 1 with a water content of 75% obtained by flocculation and concentration into a packaging bag, add 0.1mL of concentrated sulfuric acid, 0.1mL of perchloric acid, 0.05g of sodium carbonate, add 160mL of petroleum ether, and 100mL of methanol, drain Air, seal. Put the packaging bag into the container 3, increase the pressure to 400MPa, hold the pressure for 200S, increase the pressure to 500MPa, hold the pressure for 200S, and release the pressure. After taking it out, put it into a centrifuge tube and centrifuge at 3000r / min for 5min. Pour the supernatant into a separatory funnel, discard the lower water layer, take the supernatant and carry out rotary evaporation under reduced pressure, and the chlorella oil extracted by wet method is obtained after the concentration is completed.

Embodiment 3

[0068] Put 100g (about 100mL) of chlorella mud with a water content of 80% obtained by flocculation and concentration into a packaging bag, add 0.1mL of concentrated sulfuric acid, add 50mL of chloroform, and 100mL of methanol, exhaust, and seal. Put the packaging bag into the container 3, increase the pressure to 400MPa, and keep the pressure for 1200S. After taking it out, put it into a centrifuge tube, and centrifuge at 4500r / min for 18min. Pour the supernatant into a separatory funnel, add 50 mL of chloroform, shake well, and let stand for 3 h. The lower aqueous layer was discarded, and the supernatant was subjected to reduced-pressure rotary evaporation, and the chlorella oil obtained by wet extraction was obtained after the concentration was completed.

[0069] Compared with prior art, the present invention has following characteristics:

[0070] 1. There is no need to dry the chlorella, which saves a lot of heat.

[0071] 2. Microalgae cells generally have the proble...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
water contentaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method and a device for chlorella oil by virtue of a wet method. The method comprises the following steps: concentrating chlorella mud, and simultaneously adding a volatile organic solvent and an additive to the chlorella mud so as to form a mixture, wherein the additive is a strong oxidant or a combination of the strong oxidant and a carbonate agent; pressurizing the mixture to 400-600 Mpa, maintaining the pressure for a certain time, and releasing pressure; taking out the mixture subjected to pressure treatment, centrifuging, taking a liquid supernatant, discarding a lower layer of water, and evaporating and concentrating the liquid supernatant, thus obtaining the wet extraction chlorella oil. The device comprises a container, a medium storage tank and a pressurizer. The method and device for extracting the chlorella oil by virtue of the wet method can be used for solving the problems in a wet extraction process of the chlorella oil that the extraction rate of the organic solvent is low, the solvent loss is large, and the like.

Description

technical field [0001] The invention relates to a method and a device for extracting oil from chlorella, in particular to a method and a device for extracting oil from chlorella under the condition of high water content (75%-85%). Background technique [0002] At present, there are many microalgae oil extraction technologies, among which organic solvent extraction is still a relatively common method. Before extraction, due to the existence of the cell wall, it needs to be broken. Generally, methods such as swelling method, repeated freezing and thawing, low-concentration calcium chloride solution, steam high pressure, enzyme, bead mill, microwave, ultrasonic wave, and antioxidant are used for wall-breaking treatment. The organic solvents for extraction include n-hexane, isopropanol, ethanol, ether-petroleum ether, chloroform methanol, etc., and carbon dioxide is also used for supercritical extraction. Some people use microalgae powder for one-step extraction and esterifica...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/10C11B1/04
Inventor 李树君徐嘉杰赵凤敏杨炳南曹有福刘威苏丹王梅兴丽丁进锋
Owner CHINESE ACAD OF AGRI MECHANIZATION SCI
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com