Determination method of taurine content of scorpion medicinal material
A method of determination, the technology of taurine, applied in the direction of measuring devices, material separation, and analysis of materials, etc., can solve the problems of measurement result precision, poor repeatability, difficult operation, strict time control requirements, etc., and achieve specificity Strong, accurate measurement results, and quality control effects
Inactive Publication Date: 2014-07-02
SHANGHAI ZHANGJIANG ENG RES CENT OF MODERN PREPARATION TECH OF TRADITIONAL CHINESE MEDICINE
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Problems solved by technology
Wherein the UV method requires the addition of formaldehyde and taurine to measure after 1 hour in the test, and the precision and repeatability of the measurement results are not good; the HPLC method needs to carry out pre-column derivatization of taurine, and the used derivatization reagent is 2,4 - Dinitrofluorobenzene is a highly toxic compound, and the analysis and derivatization process requires extremely strict time control, making it difficult to operate
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[0015] Instruments and reagents used:
[0016] Thin-layer scanner CAMAG Scanner 3 Linomat 5 semi-automatic spotting instrument (Cama, Switzerland).
[0017] Detection wavelength: 500nm.
[0018] Silica gel G prefabricated plate Qingdao Ocean Chemical Factory; taurine reference substance (China Institute for the Control of Pharmaceutical and Biological Products); other reagents were of analytical grade (Sinopharm Group Shanghai Reagent Co., Ltd.).
[0019] linear test
[0020] Accurately weigh 11.25mg of taurine reference substance, add water to make up to 10ml, and obtain a reference substance solution with a concentration of 1.125mg / ml, respectively pipette 0.5, 1.0, 1.5, 2.0, 2.5μl and spot on the same silica gel G thin-layer plate and develop with n-butanol-glacial acetic acid-absolute ethanol-water (4:6:0.5:1), take it out, dry it in the air, bake it with 2% ninhydrin at 105°C for 5 minutes, take it out, Cover a glass plate of the same size on the thin-layer plate, fix...
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The invention discloses a determination method of taurine content of a scorpion medicinal material. The invention employs a thin-layer chromatographic scanning method, which comprises the operation steps as follows: preparing a reference substance solution by adding water into a taurine reference substance; preparing a sample solution by adding 25 ml of water into a scorpion medicinal material coarse powder, conducting reflux extraction, filtering an extract, concentrating and setting to a constant volume of 10 ml; and respectively absorbing the reference substance and the sample solution, dipping the reference substance and the sample solution on a same silica gel G thin layer plate, expanding with n-butyl alcohol, glacial acetic acid, absolute ethyl alcohol and water, taking out the thin layer plate, drying by air, baking with 2% ninhydrin at 105 DEG C for 5 minutes for coloration, taking out the thin layer plate, covering a glass plate in the same size on the thin layer plate, fixing the periphery with adhesive tapes, placing the thin layer plate at room temperature, and scanning the thin layer plate under thin layer scanner with single wavelength. The invention can effectively control the quality of the scorpion medicinal material, and can provide guarantee for original drug quality of a scorpion medicinal material finished product.
Description
technical field [0001] The invention relates to content determination of substances, in particular to a method for content determination of taurine in scorpion medicinal materials. Background technique [0002] The scorpion is the dried whole body of Buthus martensii Karsch, a species of the scorpion family, mainly produced in Henan, Shandong, Hebei and other places. Scorpion has the effects of quenching wind and relieving convulsions, attacking toxins and dispelling stagnation, dredging collaterals and relieving pain, and is used for acute and chronic convulsions, apoplexy, facial paralysis, tetanus spasm, sore swelling, intractable migraine headache, and rheumatic arthralgia. It has a wide range of clinical applications and is a relatively expensive traditional Chinese medicine. However, because it is an animal medicinal material, its components are relatively complex and difficult to separate, so there are few research documents on relevant quality standards. There is n...
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IPC IPC(8): G01N30/90
Inventor 张继全阮克锋高君伟刘春林
Owner SHANGHAI ZHANGJIANG ENG RES CENT OF MODERN PREPARATION TECH OF TRADITIONAL CHINESE MEDICINE