Method for rapidly detecting insect resistance of transgenic insect-resistant cotton
A detection method, technology of insect-resistant cotton, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as uncontrollable environmental conditions, long measurement cycle, poor test repeatability, etc., and save field management costs , low detection cost, and the effect of reducing detection cost
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[0017] (1) Get 120 uncoated seeds of cotton A, B, C, D, and E to be tested respectively, sterilize the seeds with 10% (v / v) NaClO for 20 minutes, and then wash the seeds with water for 5 times , put into 3 petri dishes, put 40 grains in each petri dish, then add water, and culture in dark at 25°C for 3 days in the incubator;
[0018] (2) Configure agar nutrient solution, dissolve ammonium nitrate (NH 4 NO 3 ) 1g, potassium dihydrogen phosphate (KH 2 PO 4 ) 8g, agar powder 3g, configure agar nutrient solution, when the temperature of the melted agar nutrient solution drops to about 50°C, pour it into a transparent culture bottle (inner diameter 8cm, height 15cm), pour into each culture bottle 250ml of agar culture solution, after the agar culture solution is solidified, cover the lid of the culture bottle;
[0019] (3) After 3 days, select cotton seeds of the same size, place them in culture bottles, place 3 seeds in each bottle, cover them, and put them in a light incubato...
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