Method for rapidly detecting insect resistance of transgenic insect-resistant cotton

A detection method, technology of insect-resistant cotton, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as uncontrollable environmental conditions, long measurement cycle, poor test repeatability, etc., and save field management costs , low detection cost, and the effect of reducing detection cost

Inactive Publication Date: 2014-07-09
AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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Problems solved by technology

[0003] The above-mentioned method for evaluating the insect resistance of transgenic cotton mainly has the following disadvantages: (1) the measurement period is too long, and generally it is necessary to measure the occurrence of cotton pests in different growth stages and different cotton bollworm stages, which takes several months; (2) Because the cotton plants are outdoors, the environmental conditions are uncontrollable, and the test repeatability is poor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] (1) Get 120 uncoated seeds of cotton A, B, C, D, and E to be tested respectively, sterilize the seeds with 10% (v / v) NaClO for 20 minutes, and then wash the seeds with water for 5 times , put into 3 petri dishes, put 40 grains in each petri dish, then add water, and culture in dark at 25°C for 3 days in the incubator;

[0018] (2) Configure agar nutrient solution, dissolve ammonium nitrate (NH 4 NO 3 ) 1g, potassium dihydrogen phosphate (KH 2 PO 4 ) 8g, agar powder 3g, configure agar nutrient solution, when the temperature of the melted agar nutrient solution drops to about 50°C, pour it into a transparent culture bottle (inner diameter 8cm, height 15cm), pour into each culture bottle 250ml of agar culture solution, after the agar culture solution is solidified, cover the lid of the culture bottle;

[0019] (3) After 3 days, select cotton seeds of the same size, place them in culture bottles, place 3 seeds in each bottle, cover them, and put them in a light incubato...

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Abstract

The invention discloses a method for rapidly detecting insect resistance of transgenic insect-resistant cotton. The method is characterized by comprising the following steps: accelerating germination of to-be-detected transgenic insect-resistant cotton seeds, seeding into a culture bottle, opening a bottle cap after cotton plants grow for 20 days to inoculate st-instar larva of cotton bollworm onto the cotton plants, and observing the death rate of larva of cotton bollworm; evaluating the insect resistance of the transgenic insect-resistant cotton: a variety in which the death rate of larva of cotton bollworm is higher than 95 percent is a high-resistant variety, a variety in which the death rate of larva of cotton bollworm is higher than 85 percent and lower than 95 percent is a moderate-resistant variety, a variety in which the death rate of larva of cotton bollworm is higher than 40 percent and lower than 85 percent is a low-resistant variety, and a variety in which the death rate of larva of cotton bollworm is lower than 40 percent is a non-insect-resistant cotton variety. Compared with the conventional method of the same type, the method has the advantages of high detection speed, low cost, easiness, high repeatability and high accuracy.

Description

technical field [0001] The invention relates to a method for quickly detecting the insect resistance of transgenic insect-resistant cotton, in particular to a method for rapidly detecting the insect resistance of transgenic insect-resistant cotton with cotton seedlings. Background technique [0002] At present, the detection methods for the insect resistance of transgenic insect-resistant cotton mainly include the following: (1) cage inoculation test method: inoculate cotton bollworms on cotton plants in cages, and observe the amount of residual cotton bollworms and the damage rate of buds and bolls (2) Field identification method: the cotton material to be tested is planted in the field, and the natural conditions are used to identify the susceptible insects. (3) Indoor identification method: during the period of pest damage, the cotton organ samples that may be damaged are taken back to the room, after treatment, cotton bollworms are inoculated, and the degree of damage of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G7/00
Inventor 赵建宁杨殿林修伟明李刚刘红梅王慧
Owner AGRO ENVIRONMENTAL PROTECTION INST OF MIN OF AGRI
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