Unlock instant, AI-driven research and patent intelligence for your innovation.

Radiation Tolerant Desert Rhodobacter and Its Application

A technology of red bacteria and radiation resistance, applied in the field of microorganisms, can solve the problems of rarely reported strains with radiation resistance and radiation resistance, and achieve the effect of promoting environmental protection

Active Publication Date: 2016-01-13
THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, few Gram-negative bacteria of the Rhodobacter genus have reported radiation-resistant strains and radiation-resistant functions.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Radiation Tolerant Desert Rhodobacter and Its Application
  • Radiation Tolerant Desert Rhodobacter and Its Application
  • Radiation Tolerant Desert Rhodobacter and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The isolation and cultivation of embodiment 1 radiation resistant desert rhodobacter

[0053] Sandy soil was collected from the rhizosphere soil of red willow on the edge of the Taklimakan Desert in Xinjiang. Weigh 1.0 g of the soil sample, put it into a triangular flask containing 99 mL of sterile water, and place it on a shaker for 20 minutes to disperse the soil evenly to form a soil suspension (10 -2 ). Use a 100 μL pipette gun to draw 100 μL of soil suspension, inject it into a centrifuge tube filled with 900 μL sterile water, blow and suck 3 times, shake evenly (10 -3 ). Then use the same method to configure a dilution of 10 -4 , 10 -5 soil bacterial suspension. Take 200 μL of a suitable concentration of bacterial suspension and spread it on an LB agar medium plate, turn the plate over and place it in an incubator at 30°C for 3 days, and then obtain the radiation-tolerant Rhodobacter desert in the present invention.

[0054] LB agar medium: 10‰ sodium chlori...

Embodiment 2

[0056] Embodiment 2 The ultraviolet tolerance test of radiation-resistant desert rhodobacter

[0057] 1. Test method

[0058]Inoculate the radiation-resistant Rhodobacter desert bacterium strain of the present invention in LB liquid medium, culture it on a shaker until the logarithmic phase of growth, collect the bacteria by centrifuging at 5000 rpm for 10 minutes at 4°C; resuspend the bacteria in an equal volume of 0.1M phosphoric acid Potassium Buffer(pH7.0)(K 2 HPO 4 27.816g, KH 2 PO 4 10.608g, dissolved in 1L distilled water, sterilized at 121°C for 20min), then centrifuged at 5000rpm at 4°C for 10min to collect the bacteria, and resuspended the bacteria again in an equal volume of 0.1M potassium phosphate buffer (pH7.0).

[0059] Place the bacterial solution in a Petri dish and stir it with a sterilized iron wire to make the bacterial cells uniform. Place the petri dishes at 0~3600J / m 2 Under different doses of ultraviolet irradiation; take the irradiated bacterial ...

Embodiment 3

[0066] Example 3 Extraction of Radiation-resistant Rhodobacter desert Rhodobacter strain 16SrDNA

[0067] The present invention also provides genome extraction, 16SrDNA amplification and sequencing methods of radiation-resistant desert rhodobacteria (R.shamoensisW402), specifically according to the following operations:

[0068] 1. Genome extraction method

[0069] Genome use of radiation-resistant desert rhodobacter according to the present invention The company's bacterial genomic DNA extraction kit (centrifugal column) for extraction, see its instructions for details.

[0070] 2. PCR amplification method

[0071] The general primer sequence used for the PCR amplification of the 16SrDNA of the radiation-tolerant Rhodobacter desert bacterium according to the present invention refers to the article of WilliamG. Weisburg (1991), and the primer is synthesized by Beijing Qingke Biotechnology Company. The kits used for the amplification were purchased from Treasure Bioengineer...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Dilution degreeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a novel bacterial strain separated from rhizosphere soil of red willow in edges of Taklimakan desert in Xinjiang Uygur Autonomous Region. The novel bacterial strain is identified to be belong to rhodobacter, and is named as Rhodobacter shamoensis W402. The novel bacterial strain possesses radiation resistance, can be use as a functional bacteria used for modification of stress environments, and can be used for constructing genetically modified organisms, and culturing stress resistant organisms.

Description

technical field [0001] The invention belongs to the technical field of microbes and relates to a new desert rhodobacter. The invention also relates to the application of the bacterium as a radiation-resistant functional strain. Background technique [0002] With the development of nuclear power technology, different degrees of nuclear leakage have caused harm to the environment and human health, which has attracted worldwide attention. Therefore, screening strains with excellent radiation tolerance and studying their DNA damage repair ability have positive significance for the repair of radiation environment. [0003] Therefore, the discovery of new radiation-resistant microorganisms has important practical significance. [0004] At present, most of the radiation-resistant microorganisms developed and studied are Gram-positive bacteria of the genus Deinococcus, such as D.radiodurans, D.gobiensis, D.radiophilus, D.radiopugnans, D.proteolyticus, D.geothermalis and D.murrayi,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01H5/00C12R1/01
Inventor 张维王琳吴港陈明林敏
Owner THE INST OF BIOTECHNOLOGY OF THE CHINESE ACAD OF AGRI SCI