Pyrethroid Pesticide Degrading Bacteria Strain and Its Bacterial Agent and Application
A technology of pesticide degradation and pyrethroids, applied in bacteria, biochemical equipment and methods, microorganisms, etc., can solve the problems of complex mechanism, low degradation efficiency of cyhalothrin, etc., and achieve good degradation effect.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0044] Example 1: Isolation and purification of Proteus mirabilis JZB42C001
[0045] The vegetable field soil samples from the suburbs of Beijing that had been applied with cyhalothrin were divided into 2 parts, 10 g each, and added to 100 mL of enrichment medium containing cyhalothrin under aseptic conditions (wherein, trifluoro The concentration of cypermethrin in the enrichment medium is 50mg / L), cultivated on a shaker at 180r / min at 30°C for 7d; then press 10% inoculum size (in this embodiment, "10% inoculum size" refers to inoculation liquid to the inoculated medium) and transfer it to the next batch of enrichment medium containing cyhalothrin (wherein, in this batch of enrichment medium, the concentration of cyhalothrin is 100mg / L), Continue to cultivate for 7d; transfer to the enrichment medium containing cyhalothrin (the concentration of cyhalothrin in the enrichment medium is 200mg / L) according to 10% inoculum size, and cultivate for 7d; then press 10 % of the inocul...
Embodiment 2
[0046] Example 2: Identification of 16SrDNA of Proteus mirabilis JZB42C001
[0047] 1. Acquisition of PCR template: it includes two methods, one is to extract genomic DNA to use it as a PCR template, and the other is to directly heat-treat the bacterial liquid to use the heat-treated bacterial liquid as a PCR template. Both methods are described below.
[0048] 1) Genomic DNA was extracted, using lysozyme plus 10% SDS method, as follows:
[0049] (a) Inoculate the purified bacterium in LB liquid medium, culture on a shaker at 180r / min at 28°C for 24h, then take 2ml of fresh culture solution, centrifuge at 12000rpm for 1-2min, and discard the supernatant ;
[0050] (b) Add 1mL, 1×TE (pH8.0) buffer to the precipitate, mix and wash, centrifuge at 12000rpm for 1-2min, and discard the supernatant;
[0051] (c) Add 400 μL, 5×TE (pH8.0) buffer to the precipitate, mix and wash, centrifuge at 12000 rpm for 1-2 min, and discard the supernatant;
[0052] (d) Add 50 μL, 20 mg / ml lysoz...
Embodiment 3
[0066] Embodiment 3: Determination of morphological characteristics and physiological and biochemical characteristics of Proteus mirabilis (Proteusmirabilis) JZB42C001 strain
[0067] The purified strain JZB42C001 was inoculated on LB solid medium, cultured at 28°C for 48 hours, and the morphological characteristics of the strain were observed by electron microscopy. The logarithmic phase of the purified strain was taken for Gram and capsule staining. The physiological and biochemical characteristics were determined according to " Handbook of Common Bacterial System Identification. The result is: the bacterial morphology is obviously pleomorphic, see image 3 , can be rod-shaped, club-shaped, spherical, filamentous, etc., size (0.4-0.6) μm × (1.0-3.0) μm, no capsule, no spore formation, flagella all over the body, Gram-negative, indole Test negative, gelatin liquefaction positive, hydrogen sulfide positive, other physiological and biochemical characteristics are shown in Tabl...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More