Bacillus licheniformis, bacillus subtilis and lactobacillus plantarum preparation and preparation

A technology of Bacillus subtilis and Bacillus licheniformis, which is applied to bacteria, microorganisms, animal feed and other directions, can solve the problems of large environmental pollution, difficult post-processing, complicated operation of machinery and equipment, etc. Body concentration, simplified fermentation equipment and easy-to-control effects

Active Publication Date: 2014-07-09
INNER MONGOLIA SCI PLUS BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The use of liquid fermentation will produce a large amount of waste water, which will cause great en

Method used

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  • Bacillus licheniformis, bacillus subtilis and lactobacillus plantarum preparation and preparation
  • Bacillus licheniformis, bacillus subtilis and lactobacillus plantarum preparation and preparation
  • Bacillus licheniformis, bacillus subtilis and lactobacillus plantarum preparation and preparation

Examples

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Example Embodiment

[0037] Example 1:

[0038] The preparation method of probiotic preparations prepared by Bacillus licheniformis, Bacillus subtilis and Lactobacillus plantarum includes bacterial culture and solid-state fermentation culture.

[0039] The solid-state fermentation culture method is as follows: firstly inoculate Bacillus licheniformis BL-09 and Bacillus subtilis HM-66 seed liquid on the solid-state fermentation medium, the inoculation amount is 3.0% (v / w) each, and the fermentation temperature is controlled at 35°C and humidity 75%, mixed bacteria culture for 12h; then add Lactobacillus plantarum HM-10 seed liquid, the inoculum amount is 1.0% (v / w), other conditions remain unchanged, and then ferment for 12h, the total fermentation time is 24h. After the fermentation, the dried culture is obtained by the fluidized bed drying method.

[0040] The solid-state fermentation medium is composed of soybean meal, bran and corn meal, and the mass ratio of the soybean meal, bran and corn meal is: ...

Example Embodiment

[0042] Example 2:

[0043] The preparation method of probiotic preparations prepared by Bacillus licheniformis, Bacillus subtilis and Lactobacillus plantarum includes strain culture and solid-state fermentation culture.

[0044] The solid-state fermentation culture method is as follows: first inoculate Bacillus licheniformis BL-09 and Bacillus subtilis HM-66 seed liquid on the solid-state fermentation medium, the inoculation amount is 3.0% (v / w) each, and the fermentation temperature is controlled at 37°C and humidity 75%, mixed bacteria culture for 12h; then add Lactobacillus plantarum HM-10 seed liquid, the inoculum amount is 1.0% (v / w), other conditions remain unchanged, and then ferment for another 24h. After the fermentation is completed, the dried product is obtained by fluidized bed drying. Cultures.

[0045] The solid-state fermentation medium is composed of soybean meal, bran and corn meal, and the mass ratio of the soybean meal, bran and corn meal is: soybean meal, bran, a...

Example Embodiment

[0047] Example 3:

[0048] The preparation method of probiotic preparations prepared by Bacillus licheniformis, Bacillus subtilis and Lactobacillus plantarum includes bacterial culture and solid-state fermentation culture.

[0049] The solid-state fermentation culture method is as follows: first inoculate Bacillus licheniformis BL-09 and Bacillus subtilis HM-66 seed liquid on the solid-state fermentation medium, the inoculation amount is 5.0% (v / w), and the fermentation temperature is controlled at 30°C and humidity 75%, mixed bacteria culture for 24h; then add Lactobacillus plantarum HM-10 seed liquid, the inoculum amount is 3.0% (v / w), other conditions remain unchanged, and then ferment for another 24h. After the fermentation is completed, the dried product is obtained by fluidized bed drying. Cultures.

[0050] The solid-state fermentation medium is composed of soybean meal, bran and corn meal, and the mass ratio of the soybean meal, bran and corn meal is: soybean meal, bran, and...

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Abstract

The invention belongs to the micro biotechnical field of feed and relates to a probiotics preparation which is prepared by solid fermentation of probiotics bacillus licheniformis BL-09, bacillus subtilis HM-66 and lactobacillus plantarum HM-10 which are mixed in stages by taking bean pulp, bran and corn flour as a matrix. According to the invention, the yield and cell concentration can be improved by adopting mixed bacteria solid state fermentation. The ability of multiple strains to yield enzymes, acids and nutritional substances and the capacity of decomposition to the matrix and the like are remarkably superior to single bacterial fermentation. The product which cannot be obtained by pure fermentation can be obtained, the preparation has the advantages of fast growing speed, high primer utilization ratio, conversion in multiple stages, stable fermented microbial population, labor and energy saving, simplified process and fermentation equipment, easiness in control and the like, and is not easy to contaminate. By fermenting soybean meal, bran and corn flour by the three bacterial strains, proteins in the soybean meal and corn flour can be decomposed to small peptides and free amino acids to facilitate digestive absorption by intestines and stomach.

Description

technical field [0001] The invention belongs to the technical field of feed microorganisms, and relates to a probiotic bacteria Bacillus licheniformis BL-09 (Bacillus licheniformis BL-09), Bacillus subtilis HM-66 (Bacillus subtilis HM- 66) and Lactobacillus plantarum HM-10 (Lactobacillus plantarum HM-10) solid-state fermentation preparation of probiotics and its preparation method can be widely used in animal microecology and other aspects. Background technique [0002] At present, most probiotic products are fermented in liquid state, and the probiotic cells are collected directly to make probiotic powder, tablet or capsule. The use of liquid fermentation will produce a large amount of waste water, which will cause great environmental pollution, difficult post-treatment, and cumbersome operation of machinery and equipment. How to obtain strains suitable for solid-state fermentation and prepare probiotic preparations with a high number of viable bacteria and good vigor will...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16C12R1/10C12R1/125C12R1/25A23K10/18
Inventor 赵树平张善亭姚国强
Owner INNER MONGOLIA SCI PLUS BIOTECH
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