Application of Nanostructured Calcium Phosphate Hollow Microspheres in Microencapsulated Culture of Microorganisms
A nanostructured, hollow microsphere technology, applied in microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of large equipment investment and long production process cycle, and achieve low equipment investment and suitable for large-scale production. , the effect of simple process
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Embodiment 1
[0019] Embodiment 1: the preparation of lactic acid bacteria microcapsules
[0020] The lactic acid bacteria seed solution was inoculated with 3% inoculum, cultured at 37°C and 110r / min for 9 hours, centrifuged at 6000r / min for 8 minutes, and nanostructured calcium phosphate hollow microspheres accounting for 2% of the total weight of microorganisms were added. Nanostructured calcium phosphate hollow microspheres The ball size is 2μm, the wall thickness is 20nm, and the high-speed homogenizer is mixed to obtain the core material; 3% sodium alginate and 1% gelatin are added to distilled water and mixed, and the embedding agent is obtained by autoclaving, and the embedding agent is cooled to After room temperature, add the core material and mix well, the volume ratio of the core material to the embedding agent is 1:2, pass through the Equl microcapsule generator, and add 8g / l CaCl dropwise 2 , solidify for 40min, and collect the lactic acid bacteria microcapsules by filtration. ...
Embodiment 2
[0021] The preparation of embodiment 2 butyric acid bacteria microcapsules
[0022] Inoculate the butyric acid bacteria seed solution with 3% inoculation amount, culture at 37°C and 120r / min for 10h, centrifuge at 8000r / min for 5 minutes, add nanostructured calcium phosphate hollow microspheres accounting for 3% of the total weight of microorganisms, and nanostructured calcium phosphate hollow microspheres The microsphere size is 4μm, the wall thickness is 500nm, and the high-speed homogenizer is mixed to obtain the core material; 3% sodium alginate and 1% gelatin are added to distilled water and mixed, and the embedding agent is obtained by autoclaving, and the embedding agent is cooled. After reaching room temperature, add the core material and mix well, the volume ratio of the core material to the embedding agent is 1:3, pass through the Equl microcapsule generator, and add 10g / l CaCl dropwise 2 , solidified for 50 minutes, and filtered to collect butyric acid bacteria micr...
Embodiment 3
[0023] The preparation of embodiment 3 Enterococcus faecium microcapsules
[0024] Inoculate the seed solution of Enterococcus faecium at 3% inoculation amount, culture at 37°C and 120r / min for 10h, centrifuge at 8000r / min for 5 minutes, add nanostructured calcium phosphate hollow microspheres accounting for 4% of the total weight of microorganisms, nanostructured calcium phosphate Hollow microspheres with a size of 3 μm and a wall thickness of 200 nm were mixed with a high-speed homogenizer to obtain a core material; 3% sodium alginate and 1% gelatin were added to distilled water and mixed, and sterilized by high pressure to obtain an embedding agent, embedding agent After cooling to room temperature, add the core material and mix well, the volume ratio of the core material to the embedding agent is 1:4, pass through the Equl microcapsule generator, and add 9g / l CaCl dropwise 2 , solidified for 40 minutes, and collected Enterococcus faecium microcapsules by filtration.
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