Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for preparing Guangchang Bailian hypotensive polypeptide by microbial fermentation

A technology for microbial fermentation and lowering blood pressure, applied in the field of peptide extraction from natural resources, can solve problems such as high cost and complex production process

Active Publication Date: 2016-08-17
清枫链食苏打饮品(吉林)有限公司
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for obtaining white lotus polypeptide with high blood pressure lowering activity in view of the two problems existing in the acquisition of Guangchang white lotus antihypertensive polypeptide, that is, high cost and complicated production process

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing Guangchang Bailian hypotensive polypeptide by microbial fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The white lotus is ground into powder, and the formula (kg / L) of the fermentation medium is: 4% white lotus powder, 3% glucose, the rest is water, and the natural pH value. Take the activated strain with a bacterial age of 36 hours, inoculate the Bacillus licheniformis N16 seed liquid in the fermentation broth medium at a 5% inoculum amount, and culture it on a shaker at 37°C and 125 r / min for 46 hours. The fermentation broth was taken, centrifuged at 10000r / min for 20min, and the precipitates were bacterial cells and unhydrolyzed white lotus components. The supernatant was filtered through a microporous membrane (0.45μm), and the filtrate was vacuum-dried. After dissolving in distilled water, separate and purify it under Sephadex-G25, according to the OD of each collection tube 220 , four absorption peaks appeared in the fermentation product after gel chromatography. The second absorption peak was collected for vacuum drying. Its ACE inhibition rate was 64.13%.

Embodiment 2

[0017] The white lotus is ground into powder, and the formula (kg / L) of the fermentation medium is: 5% white lotus powder, 2% glucose, the rest is water, and the natural pH value. Take the activated strain with a bacterial age of 38 hours, inoculate the Bacillus licheniformis N16 seed liquid in the fermentation medium according to the inoculum amount of 4%, and cultivate it on a shaking table at 37°C and 125r / min for 42 hours. The fermentation broth was taken, centrifuged at 10000r / min for 20min, and the precipitates were bacterial cells and unhydrolyzed white lotus components. The supernatant was filtered through a microporous membrane (0.45μm), and the filtrate was vacuum-dried. After dissolving in distilled water, separate and purify it under Sephadex-G25, according to the OD of each collection tube 220 , the fermentation product appeared three absorption peaks after gel chromatography. The second absorption peak was collected for vacuum drying. Its ACE inhibition rate wa...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for preparing Guangchang white lotus seed antihypertensive polypeptide through microbial fermentation. The method comprises steps of grinding white lotus into powder, wherein a fermentation liquor culture medium comprises following components (kg / L): 3-6% of white lotus powder, 1-5% of carbon source and water, and has natural pH value; taking activated bacterial strain with cell age of 28-44 hours, diluting bacillus licheniformis ND till 1mL of bacillus licheniformis ND comprises 1*107CFU, and then inoculating a seed solution into the fermentation liquor culture medium according to an inoculating amount of 2-6%, shake cultivating for 32-52 hours at 37DEG C at the speed of 125rpm; taking the fermentation liquor, centrifuging for 20 minutes at the speed of 10000rpm, wherein the precipitate is thallus and unhydrolyzed white lotus component, taking the supernate, filtering by a 0.45mu m millipore filter membrane, vacuum drying the filtrate; dissolving by distilled water, then separating and purifying through Sephadex-G25, according to the OD220 of each collecting tube, collecting the second absorption peak after chromatography of fermentation products by gel, and vacuum drying. The method is simple and has low cost; most mixed polypeptide prepared by the method has the molecular weight less than 1500D, and the polypeptide prepared by the method is better than the polypeptide prepared from papain in antihypertensive activity.

Description

technical field [0001] The invention belongs to the extraction of peptides in natural resources, in particular to the extraction of blood pressure-lowering active peptides. technical background [0002] Angiotensin Converting Enzyme (ACE) can increase blood pressure. Substances that can inhibit the activity of ACE are called angiotensin converting enzyme inhibitors (Angiotensin Converting Enzyme inhibitor, ACEI), studies have found that many natural proteolytic peptides can inhibit the activity of ACE. These peptides have great potential for development in the prevention and treatment of hypertension. Food-derived protein antihypertensive peptides can be developed into antihypertensive drugs or antihypertensive health products due to their non-toxicity, no side effects, and high safety. [0003] Guangchang white lotus has the characteristics of high protein, low fat, and rich amino acid content. It is an excellent health food for both medicine and food. Through the resear...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16C12R1/10
Inventor 吴丽萍段元丹李恒袁建赣李曼
Owner 清枫链食苏打饮品(吉林)有限公司