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Method for shape representation of hydrogel micro-pore arrays

A technology of microwell array and morphology characterization, applied in scanning probe microscopy, measuring devices, instruments, etc., to achieve the effect of high precision and high spatial resolution

Active Publication Date: 2014-07-09
XI AN JIAOTONG UNIV
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Problems solved by technology

Based on the working principle and the above advantages of scanning electrochemical microscopy, it is speculated that its application in the characterization of hydrogel microwell arrays is feasible, but there are no related reports so far

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  • Method for shape representation of hydrogel micro-pore arrays
  • Method for shape representation of hydrogel micro-pore arrays
  • Method for shape representation of hydrogel micro-pore arrays

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Embodiment Construction

[0039] The present invention will be described in more detail below in conjunction with the accompanying drawings and specific embodiments.

[0040] Instruments and reagents used in this example:

[0041] Polyethylene glycol dimethacrylate (relative molecular weight is about 1000g / mol, Polysciences Inc. USA), 2-hydroxy-2-methylpropiophenone (molecular weight is 164.2g / mol, Tokyo Chemical Industry Co., Japan) , potassium chloride (≥99.5%, Sigma-Aldrich, USA), sodium hydroxide (≥98.0%, Sinopharm Group, Shanghai), absolute ethanol (≥99.7%, Zhiyuan Chemical Reagent Co., Ltd., Tianjin). Polycarbonate photomask (Qingyi Optoelectronics Co., Ltd., Shenzhen), polydimethylsiloxane template (SYLGARD184, Dow Corning, USA), and cover glass (Jiangsu Feizhou Glass & Plastic Co., Ltd.). All aqueous solutions used in the experiment were prepared with ultrapure water prepared by Milli-Q Reference System (Millipore, USA). Scanning electrochemical microscope (CHI920C, Shanghai Chenhua), ultravi...

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Abstract

A method for shape representation of hydrogel micro-pore arrays comprises the steps that firstly, a selected hydrogel material is polyethylene glycol dimethacrylate and an optical masking method and a template method are adopted for preparing the polyethylene glycol dimethacrylate hydrogel micro-pore arrays with different pore diameters and different pore depths; secondly, a scanning electrochemical microscope probe is prepared and a scanning electrochemical microscope detection system is established; finally, home position representation is conducted on the shape of the polyethylene glycol dimethacrylate hydrogel micro-pore arrays in an aqueous solution through a scanning electrochemical microscope. According to the method, natural oxygen couples in the aqueous solution serve as redox couples, home position representation is conducted on the shape of the polyethylene glycol dimethacrylate hydrogel micro-pore arrays in the aqueous solution through the scanning electrochemical microscope technology, two-dimensional pore diameter and three-dimensional shape information of the surfaces of the hydrogel micro-pore arrays is obtained, and the method has the advantages that home position representation, reversible representation and lossless representation can be conducted on a sample in the aqueous solution and the three-dimensional shape information of the hydrogel material can be obtained.

Description

technical field [0001] The invention relates to the technical field of hydrogel characterization, in particular to a method for characterizing the morphology of a hydrogel micropore array. Background technique [0002] Hydrogel microwell array is a new type of substrate soft material for cell culture, which has the following advantages: (1) a three-dimensional network structure with high water content similar to extracellular matrix is ​​conducive to cell proliferation and spreading; (2) physical , The chemical properties are easy to adjust, which can meet the special requirements of cell culture; (3) High transparency, which is beneficial to observe cells during cell culture. Therefore, hydrogel microwell arrays have shown broad application prospects in cell culture, which has aroused widespread interest. [0003] The microwell morphology of the hydrogel microwell array has a direct impact on the behavior of the cells cultured in it, so the characterization of the hydrogel...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01Q60/60G01B21/20
Inventor 李菲陈咏梅徐峰高扬杜晓静
Owner XI AN JIAOTONG UNIV
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