11 results about "Scanning electrochemical microscopy" patented technology

The invention discloses a scanning electrochemical microscope in-situ stress corrosion testing device and method, and belongs to the technical field of material stress corrosion testing, and the device comprises a base, a power transmission module, a clamping module, a corrosion module, a signal acquisition module and a scanning electrochemical microscope module. Slow strain rate stretching of a sample is achieved through a servo motor, a planetary gear speed reducer and a transmission mechanism, the clamping module is integrated with a force sensor to monitor loads in real time, the corrosion module provides a temperature-controllable corrosion environment, and the scanning electrochemical microscope module achieves electrochemical scanning of the surface of the sample. The device can synchronously obtain a load-deformation curve, corrosion morphology and microcell electrochemical information under stress-corrosion-temperature multi-field coupling, and provides an effective technical means for deeply researching a stress corrosion mechanism of a material. The method comprises the steps of sample installation, corrosion solution preparation, electrode arrangement, tensile test, electrochemical scanning and the like, and has the advantages of simplicity and convenience in operation, comprehensive data, high precision and the like.

The use of the scanning electrochemical microscopy to predict the corrosion resistance results which would be obtained for a surface S1 having undergone an anticorrosion treatment if the surface S1 was subjected to a salt fog corrosion test, which use comprises an analysis of the surface S1 by scanning electrochemical microscopy.

The invention discloses a micrometer standard sample plate for a scanning electrochemical microscope, a matched clamping and connecting device and a length calibration method, and belongs to the technical field of precise instrument calibration. The sample plate comprises a monocrystalline silicon substrate, a micron-sized standard pattern structure formed on the surface of the monocrystalline silicon substrate, and a conductive film layer covering the surface of the micron-sized standard pattern structure. And a mounting table matched with the clamping connecting device is arranged in the center of the upper surface of the base. The length calibration method comprises the following steps: installing and fixing the micrometer standard sample plate assembly on a sample table of the scanning electrochemical microscope, and carrying out electric connection conduction; the X direction and the Y direction are calibrated; and correcting the length measurement parameters of the scanning electrochemical microscope. The micron standard sample plate provided by the invention has good conductivity and stable geometrical morphology. The matched clamping and connecting device can realize rapid and accurate positioning of the standard sample plate on the scanning electrochemical microscope sample table. The calibration method ensures the accuracy and traceability of the measurement value of the equipment.

This invention relates to the field of electrochemical technology, and more particularly to a method for testing the reaction kinetics at the Bi electrode interface in Li-Bi batteries. The dual-probe measurement system constructed in this invention works collaboratively on a precise three-dimensional positioning platform of a shared scanning electrochemical microscope. One probe maps the electrochemical activity distribution on the electrode surface, visually reflecting the spatial location of low-activity phases, while the other Hg probe simultaneously records the Li content in the corresponding region. + Concentration or diffusion coefficient. This "functionally complementary, spatially synchronized" measurement mode can integrate observed electrochemical data, interfacial electrochemical activity, and Li... + The migration dynamics are directly related, thus providing experimental evidence for revealing the true rate-limiting steps in the phase transition process, enabling a more comprehensive and three-dimensional analysis of complex electrode processes, and making up for the limitations of single signal measurement.

The invention discloses an optical fiber probe structure for micro-nano scale sensing and control, and belongs to the technical field of micro-nano manufacturing and the field of micro-nano sensing and control. The optical fiber probe is composed of a cladding, a light guide core and a conductive core, the light guide core is located in the center of the cladding, the probe structure comprises the optical fiber probe, the optical fiber probe comprises the cladding and the light guide core in the center, the conductive core is further arranged in the cladding, the front end of the optical fiber probe is tapered to be a sensing part, and the rear end of the optical fiber probe is a connecting part for transmitting optical signals and electric signals. The sensing part and the conductive core and the light guide core in the sensing part are all provided with micro-nano-level tips. And the tail end of the connecting part is simultaneously connected with a common optical fiber and a wire for transmitting an optical signal and an electric signal. The probe is simple and changeable in structure and diversified in function; in combination with an atomic force microscope, the temperature, surface topography image and optical image information of the sample can be obtained at the same time; meanwhile, the method can be applied to scanning electrochemical microscopes, cell activity state monitoring, micro-nano control and thin film material characteristic detection.

The present application relates to the technical field of aqueous ion battery, and particularly relates to a method for testing electrochemical activity uniformity of negative electrode material of aqueous ion battery. The method comprises the following steps: preparing a sample pool, wherein the sample pool comprises a substrate layer and a negative electrode material layer to be tested on the substrate; placing the sample pool in a photo-assisted scanning electrochemical microscope system, and performing leveling, wherein the photo-assisted scanning electrochemical microscope system comprises a scanning electrochemical microscope system and a side-viewing CCD camera; and calculating the electrochemical activity uniformity of the surface of the negative electrode material to be tested. The method has the advantages of high speed and high precision by using the photo-assisted scanning electrochemical microscope system for leveling. The method does not depend on the assumption of the electrochemical activity uniformity of the surface to be tested in the actual operation of the traditional SECM system. The leveling time is less than 10 minutes each time, which is much less than 30 minutes required by the ordinary approximation curve leveling method. Therefore, the electrochemical property detection method in the present application is suitable for large-scale electrode screening, and has good application prospect.

The present application relates to a method for detecting the degree of irreversible electroporation by scanning electrochemical microscopy (SECM), which realizes rapid and efficient detection of the degree of cell electroporation by measuring the membrane permeability (Pm) of cells after stimulation by a pulsed electric field. The method first measures the approach curve of the SECM probe to the cells, then converts the approach curve into the membrane permeability of the cells to complete the measurement of the degree of electroporation, and measures the survival rate curve of the cells by the existing detection method, establishes the relationship between the Pm value and the cell survival rate, verifies the detection of irreversible electroporation by the membrane permeability of the cells, and finds that the law of the change of the membrane permeability with the pulsed voltage is almost the same as the law of the change of the cell survival rate with the pulsed voltage, so the method for measuring the degree of cell electroporation by SECM is effective and reliable.

The invention belongs to the technical field of scanning electrochemical microscopes, and discloses a bacterial biofilm metabolic activity detection method based on a scanning electrochemical microscope technology and a microarray, and the method comprises the following steps: inoculating bacteria to be detected into microarray holes, applying an antibacterial agent for incubation, the method comprises the following steps: adding a phosphate buffer solution containing resazurin, ferrocene methanol and glucose for determination, positioning an SECM probe to a fixed height position on the surface of a microarray, and carrying out global scanning on the whole microarray in a line scanning manner to obtain a reduction current signal measured by an electrode. According to the method, the metabolic activity of the biological membrane can be accurately indicated, and misjudgment and background color interference generated when an optical signal of a resazurin reduction product is used for detection can be avoided. The metabolic activity change of the biological membrane before and after antibacterial agent treatment can be researched and represented so as to assist in screening and development of the antibacterial agent, and the method can also be used for biological membrane pollution detection in the environment.

The application discloses a kind of microbial photoelectrochemical characterization device and use method, microbial photoelectrochemical characterization device includes: the support with scanning station;Multi-cavity sample cell, rotatably arranged on support, including transparent base electrode and side wall structure, side wall structure and base electrode are enclosed to form multiple annular distribution containing cavities;Illumination device, arranged on support and used to provide illumination;Scanning electrochemical microscope device, including three-dimensional controller, electrochemical workstation, ultramicroelectrode, reference electrode and counter electrode;Cover, cover is arranged on the top of multi-cavity sample cell, cover is correspondingly provided with first through-hole for passing ultramicroelectrode, reference electrode and counter electrode on each containing cavity;Gas supply device, for providing nitrogen;Driving device, for driving multi-cavity sample cell rotation.The structure can make sample in the process of scanning test in anaerobic state, and scanning test can also be carried out to multiple samples.

This invention belongs to the field of bioelectrochemical detection and cell interface characterization technology, specifically relating to a real-time dynamic in-situ detection system and method for cell micro-region impedance based on scanning electrochemical microscopy. The system includes: a conductive substrate electrode, a SECM probe, a cell culture module, a multi-channel electrochemical workstation, and a control system and imaging software. By precisely scanning near the cell surface using the SECM probe, impedance response signals are acquired simultaneously with the application of alternating excitation signals, enabling real-time dynamic in-situ detection of impedance in micro-regions on the surface of living cells, achieving sub-micron spatial resolution. This invention integrates impedance detection and electrochemical detection functions, enabling label-free, non-invasive continuous monitoring during cell culture, providing a novel high-resolution, multi-parameter synchronous acquisition analytical platform for cell physiological state research, drug screening, and pathological mechanism analysis.

The invention discloses a single-cell NO electrochemical sensor based on a fluorinated monomolecular layer modified nanotube electrode and a preparation method of the single-cell NO electrochemical sensor, and belongs to the field of electrochemical sensors. The preparation method comprises the following steps: firstly, drawing a borosilicate glass tube into a 200nm tip by a laser drawing instrument, then sputtering a conductive gold layer on the surface of the glass tube by plasma sputtering, insulating the redundant part by PDMS, then manufacturing a hollow nanotube electrode by a connecting wire, and finally self-assembling on the surface of the electrode by a gold-sulfur bond to form a fluorinated monomolecular layer. The nanotube electrode modified with perfluorodecanethiol has good selectivity to NO, can accurately detect NO in a single cell under physiological conditions, and is low in detection limit, short in response time and high in anti-interference capability. According to the invention, the scanning ion conductance microscope and the scanning electrochemical microscope are combined, the cell morphology can be imaged while detection is carried out, and the method is expected to play an important role in the field of biomedical analysis and detection and has commercial potential.