A method for characterizing the morphology of hydrogel microwell arrays

A technology of micropore array and topography characterization, which is used in scanning probe microscopy, measuring devices, instruments, etc. to achieve the effect of high precision and high spatial resolution

Active Publication Date: 2017-02-08
XI AN JIAOTONG UNIV
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Problems solved by technology

Based on the working principle and the above advantages of scanning electrochemical microscopy, it is speculated that its application in the characterization of hydrogel microwell arrays is feasible, but there are no related reports so far

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  • A method for characterizing the morphology of hydrogel microwell arrays
  • A method for characterizing the morphology of hydrogel microwell arrays
  • A method for characterizing the morphology of hydrogel microwell arrays

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[0039] The present invention will be described in more detail below in conjunction with the accompanying drawings and specific embodiments.

[0040] Instruments and reagents used in this example:

[0041] Polyethylene glycol dimethacrylate (relative molecular weight is about 1000g / mol, Polysciences Inc. USA), 2-hydroxy-2-methylpropiophenone (molecular weight is 164.2g / mol, Tokyo Chemical Industry Co., Japan) , potassium chloride (≥99.5%, Sigma-Aldrich, USA), sodium hydroxide (≥98.0%, Sinopharm Group, Shanghai), absolute ethanol (≥99.7%, Zhiyuan Chemical Reagent Co., Ltd., Tianjin). Polycarbonate photomask (Qingyi Optoelectronics Co., Ltd., Shenzhen), polydimethylsiloxane template (SYLGARD184, Dow Corning, USA), and cover glass (Jiangsu Feizhou Glass & Plastic Co., Ltd.). All aqueous solutions used in the experiment were prepared with ultrapure water prepared by Milli-Q Reference System (Millipore, USA). Scanning electrochemical microscope (CHI920C, Shanghai Chenhua), ultravi...

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Abstract

A method for characterizing the morphology of hydrogel micropore arrays. The hydrogel material selected first is polyethylene glycol dimethacrylate, and photomask method and template method are used to prepare The polyethylene glycol dimethacrylate hydrogel microwell array; then prepare the scanning electrochemical microscope probe and build the scanning electrochemical microscope detection system; finally apply the scanning electrochemical microscope to detect the polyethylene glycol dimethacrylate in the aqueous solution In situ characterization process of ester hydrogel microporous array morphology. In the present invention, the natural electron pair oxygen in the aqueous solution is used as the reducing pair, and the morphology of the polyethylene glycol dimethacrylate hydrogel micropore array in the aqueous solution is characterized in situ by using scanning electrochemical microscopy technology, and the water The two-dimensional pore size and three-dimensional shape information on the surface of the gel micropore array has the advantages of in-situ, reversible and non-destructive characterization of the sample in aqueous solution and gives the three-dimensional shape information of the hydrogel material.

Description

technical field [0001] The invention relates to the technical field of hydrogel characterization, in particular to a method for characterizing the morphology of a hydrogel micropore array. Background technique [0002] Hydrogel microwell array is a new type of substrate soft material for cell culture, which has the following advantages: (1) a three-dimensional network structure with high water content similar to extracellular matrix is ​​conducive to cell proliferation and spreading; (2) physical , The chemical properties are easy to adjust, which can meet the special requirements of cell culture; (3) High transparency, which is beneficial to observe cells during cell culture. Therefore, hydrogel microwell arrays have shown broad application prospects in cell culture, which has aroused widespread interest. [0003] The microwell morphology of the hydrogel microwell array has a direct impact on the behavior of the cells cultured in it, so the characterization of the hydrogel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01Q60/60G01B21/20
Inventor 李菲陈咏梅徐峰高扬杜晓静
Owner XI AN JIAOTONG UNIV
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