Pesticidal composition containing clothianidin and fipronil and application thereof in preventing and treating soil insects
An insecticidal composition and technology for subterranean pests, applied in the field of pesticides, can solve problems such as difficulty in effectively suppressing subterranean pests and limited control methods, and achieve the effects of reducing the amount of pesticides used, improving drug efficacy, and reducing environmental pressure
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Embodiment 1
[0042] The purpose of this example is to study the synergistic effect of different weight ratios of clothianidin and fipronil on the control of North China black beetle.
[0043] The indoor toxicity of the test agents to the 2nd instar larvae of the North China black gill beetle was soaked in insects. Dilute the mother liquors of each agent with an aqueous solution containing 0.1% Tween 80 to form 4 to 6 series of mass concentrations in an equiproportional gradient. Distilled water containing 0.1% Tween 80 and 0.1% acetone was used as blank control. Pick the 2nd instar larvae of the North China black beetle, which are healthy, lively and in the same physiological state, and put them in the dipping cage, soak them in the liquid medicine for 30 seconds, absorb the excess liquid medicine with absorbent paper, and put them into the feed as the finger food. Type tube (diameter 1.8cm, length 8cm), sealed with a cotton plug. Place them at a temperature of 24-26°C and a relative hum...
Embodiment 2
[0051] The purpose of this example is to study the synergistic effect of clothianidin and fipronil mixed in different weight ratios on the needleworm.
[0052] The indoor virulence of the test agents on the 3rd instar larvae of Chrysalis fine-breasted adopts dipping method. Dilute the mother liquors of each agent with an aqueous solution containing 0.1% Tween 80 to form 4 to 6 series of mass concentrations in an equiproportional gradient. Distilled water containing 0.1% Tween 80 and 0.1% acetone was used as blank control. Put the test insects into the funnel folded with filter paper, take 3mL of the above liquid medicine into the funnel with a pipette gun, keep the test insects in the liquid medicine for 30 seconds, take them out and put them on the filter paper to absorb the excess liquid medicine , put the test insects into a glass insect culture tube (diameter 1.8cm, height 8cm), put an appropriate amount of water content (about 18%) wet soil and an appropriate amount of n...
Embodiment 3
[0060] The indoor toxicity of the test agents to the 2nd instar larvae of the North China black gill beetle was soaked in insects. The mother solutions of each agent were diluted with an aqueous solution containing 0.1% Tween 80 to the concentration to be tested. Distilled water containing 0.1% Tween 80 and 0.1% acetone was used as blank control. Pick the 2nd instar larvae of the North China black beetle, which are healthy, lively and in the same physiological state, and put them in the dipping cage, soak them in the liquid medicine for 30 seconds, absorb the excess liquid medicine with absorbent paper, and put them into the feed as the finger food. Type tube (diameter 1.8cm, length 8cm), sealed with a cotton plug. Place them at a temperature of 24-26°C and a relative humidity of 60-80%, and raise and observe them without light. Investigate the death situation of test worm after 5 days, the standard of death is that the skilled technician touches the worm body lightly with a...
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