Test strip for specifically measuring IgM, IgG and IgA blood group antibodies

Abstract

The invention belongs to the field of biological medicines and discloses an immunochromatography test paper technology, which is used for specifically measuring the negative, positive and titer of IgM, IgG and IgA blood group antibodies in blood or body fluid or tissue fluid or secretion of people. The technology comprises the following steps: (1) preparing a sample filter membrane and a sample pad; (2) preparing a tracer and a tracing pad; (3) preparing a fiber membrane detection area and a quality control area; (4) labeling a test strip; (5) assembling the test strip; (6) injecting a sample and detecting; and (7) reading a result. The test strip can be used for measuring IgM anti-A and anti-B antibody titer, monitoring implantation of bone marrow transplantation and measuring the titer of immune IgG anti-A and anti-B antibodies of individuals, particularly prenatal pregnant women and newborns, the reverse typing of blood groups can be accurately judged by specifically measuring the IgM anti-A and anti-B antibodies, and the reverse typing of the blood group of an individual can be judged through saliva without blood sampling by specifically measuring IgA anti-A and anti-B antibodies in secretion.

Description

technical field [0001] The invention belongs to the field of biomedicine, and discloses a method for specifically measuring IgM, IgG, and IgA anti-A and anti-B blood group antibodies and their potency using immunochromatographic test paper technology, which can be used for determining IgM anti-A and anti-B blood group antibodies. -B antibody titer monitoring of bone marrow transplantation, determination of individual, especially pregnant women's prenatal and neonatal immune IgG anti-A and anti-B antibody titers, specific determination of IgM anti-A and anti-B antibodies can be accurate To judge the anti-typing of blood type, the specific determination of IgA anti-A and anti-B antibodies in the secretion fluid can judge the anti-typing of individual blood type through saliva without blood collection. Background technique [0002] Immunochromatography test paper technology has been widely used in the detection of various infectious disease factors, antibodies, hormones, enzyme...

AI Technical Summary

Problems solved by technology

When specifically measuring IgM, it can be used for blood type reverse determination, which will be synchronized with the agglutination method (agglutination method for the determination of IgM antibodies), eliminate the interference of IgG and other antibodies, reduce the HOOK effect, and greatly improve the accuracy and stability of the measurement results; specificity When measuring the titer of IgM antibody, it can monitor whether the implantation of bone marrow transplantation is successful, which will be the simplest and most direct proof, and can also be used in the scientific research of blood transfusion medicine; Titer or semi-quantitative or quantitative value, can be used for the determination of individual, especially pregnant women and neonatal immune anti-A or anti-B antibody titer, to prevent ABO blood type hemolytic disease of newborns (HDN); For IgA anti-A and anti-B antibodies, the anti-typing of the individual's blood type can be determined through secretions such as saliva, without collecting blood samples, and without special treatment of saliva, which can make blood type determination into home self-test or self-test (DIY), while about 20% of the non-secretory individuals with saliva blood type cannot be detected in the past

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