Test strip for specifically measuring IgM, IgG and IgA blood group antibodies

A blood group antibody and specific technology, which is applied in the field of test strips for the specific determination of IgM, IgG and IgA blood group antibodies, can solve the problem of reducing the HOOK effect and stability; when the IgM antibody titer is specifically determined, it can monitor bone marrow transplantation Whether the implantation is successful will be the simplest and most direct proof, and it can also be used for scientific research in blood transfusion medicine; when specifically determining IgG blood group antibodies, the titer or semi-quantitative or quantitative value of IgG antibodies can be determined, which can be used for individual Especially in pregnant women and neonates immune anti-A or anti-B antibody titers and other issues

Inactive Publication Date: 2014-07-16
邵超鹏
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When specifically measuring IgM, it can be used for blood type reverse determination, which will be synchronized with the agglutination method (agglutination method for the determination of IgM antibodies), eliminate the interference of IgG and other antibodies, reduce the HOOK effect, and greatly improve the accuracy and stability of the measurement results; specificity When measuring the titer of IgM antibody, it can monitor whether the implantation of bone marrow transplantation is successful, which will be the simplest and most direct proof, and can also be used in the scientific research of blood transfusion medicine; Ti

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  • Test strip for specifically measuring IgM, IgG and IgA blood group antibodies

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Embodiment 1

[0023] Embodiment 1: An immunochromatographic test strip method for measuring anti-A and anti-B antibody titers in pregnant women's serum or plasma, the main steps are as follows.

[0024] 1. Production of sample filter membrane: including a "specific component filter membrane", select nitrocellulose membrane, and saturate and embed anti-human IgM antibody.

[0025] 2. Production of tracers and tracer pads: tracers include tracer particle conjugates and tracer particles, tracer particles are selected from colloidal gold, tracer particle conjugates are goat anti-human IgG antibodies, and the tracer is dispersed and adsorbed Freeze-dried on a glass fiber pad to form a tracer pad.

[0026] 3. Preparation of fiber membrane detection area and quality control area: the fiber membrane is made of nitrocellulose membrane, using 2 fiber membranes, one with 4 detection lines, respectively embedded blood group antigen A; the other 1 fiber membrane is also equipped with 4 detection line...

Embodiment 2

[0031] Embodiment 2: An immunochromatographic test strip assay method using finger blood to assay IgM-like ABO blood group antibodies, the main steps are as follows.

[0032] 1. Fabrication of sample filter membrane: including 2 types, the first one is "formed component filter membrane", which uses glass fiber membrane to prevent red blood cells and white blood cells in the sample from entering the detection area; the second one is "specific component filter membrane", which is selected Nitrocellulose membrane, protein A embedded.

[0033] 2. The making of tracer and tracer pad: tracer comprises tracer particle conjugate and tracer particle, tracer particle selects colloidal gold for use, tracer particle conjugate is mouse source IgM anti-A and anti-B antibody, will The tracer is dispersed and adsorbed on the glass fiber pad and freeze-dried to form a tracer pad.

[0034] 3. Preparation of fiber membrane detection area and quality control area: nitrocellulose membrane was...

Embodiment 3

[0038] Embodiment 3: An immunochromatographic test strip method using saliva to determine ABO blood group reverse typing, the main steps are as follows.

[0039] 1. Fabrication of sample filter membrane: The above-mentioned "formed component filter membrane" and "specific component filter membrane" are not required.

[0040] 2. Production of tracers and tracer pads: tracers include tracer particle conjugates and tracer particles, the tracer particles are selected from colloidal gold, and the tracer particle conjugates are mouse anti-human IgA antibodies, and the tracer is dispersed and adsorbed Freeze-dried on a glass fiber pad to form a tracer pad.

[0041] 3. Preparation of the fiber membrane detection area and quality control area: the fiber membrane is made of nitrocellulose membrane, and two detection lines are set to embed blood group antigens A and B respectively, and the quality control area is embedded with anti-mouse anti-human IgA antibody.

[0042] 4. Assembly...

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Abstract

The invention belongs to the field of biological medicines and discloses an immunochromatography test paper technology, which is used for specifically measuring the negative, positive and titer of IgM, IgG and IgA blood group antibodies in blood or body fluid or tissue fluid or secretion of people. The technology comprises the following steps: (1) preparing a sample filter membrane and a sample pad; (2) preparing a tracer and a tracing pad; (3) preparing a fiber membrane detection area and a quality control area; (4) labeling a test strip; (5) assembling the test strip; (6) injecting a sample and detecting; and (7) reading a result. The test strip can be used for measuring IgM anti-A and anti-B antibody titer, monitoring implantation of bone marrow transplantation and measuring the titer of immune IgG anti-A and anti-B antibodies of individuals, particularly prenatal pregnant women and newborns, the reverse typing of blood groups can be accurately judged by specifically measuring the IgM anti-A and anti-B antibodies, and the reverse typing of the blood group of an individual can be judged through saliva without blood sampling by specifically measuring IgA anti-A and anti-B antibodies in secretion.

Description

technical field [0001] The invention belongs to the field of biomedicine, and discloses a method for specifically measuring IgM, IgG, and IgA anti-A and anti-B blood group antibodies and their potency using immunochromatographic test paper technology, which can be used for determining IgM anti-A and anti-B blood group antibodies. -B antibody titer monitoring of bone marrow transplantation, determination of individual, especially pregnant women's prenatal and neonatal immune IgG anti-A and anti-B antibody titers, specific determination of IgM anti-A and anti-B antibodies can be accurate To judge the anti-typing of blood type, the specific determination of IgA anti-A and anti-B antibodies in the secretion fluid can judge the anti-typing of individual blood type through saliva without blood collection. Background technique [0002] Immunochromatography test paper technology has been widely used in the detection of various infectious disease factors, antibodies, hormones, enzyme...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/532G01N33/544
CPCG01N33/80G01N33/558
Inventor 邵超鹏
Owner 邵超鹏
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