A kind of preparation method of corn and eucommia leaf lactase for livestock and poultry
A technology for producing eucommia leaves and lactase, which is applied in the field of lactase production, can solve problems such as no major improvement, achieve significant economic and social benefits, reduce energy consumption, and reduce consumption
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Embodiment 1
[0026] The first is the preparation of 1000ml third-stage Streptococcus lactis fermentation broth
[0027] 1) Culture medium preparation:
[0028] ①Weigh 30g of peptone, 3g of beef extract, 3.5g of sodium chloride, 30g of lactose or glucose, and 18g of calcium carbonate;
[0029] ②Put the weighed peptone, beef extract, and sodium chloride into 500ml of pure water, heat to dissolve, adjust the pH to 6.8, and then replenish to 1500ml with pure water;
[0030] ③ Distribute the above-prepared medium into Φ15×150mm test tubes (9ml each), 250ml Erlenmeyer flasks (150ml each), and 1000ml Erlenmeyer flasks (750ml each), and add weighed lactose or Glucose, wrapped up and placed in a sterilizing pot, sterilized at 119°C for 28 minutes, cooled for later use.
[0031] 2) Preparation of fermentation broth:
[0032] Take a slant for production, transfer Streptococcus lactis into the first-level test tube at 38°C for 22 hours according to the operation requirements of the inclined-plane t...
Embodiment 2
[0039] Preparation of 100L third-stage Streptococcus lactis fermentation broth:
[0040] 1) Preparation of primary, secondary and tertiary medium:
[0041] ①Weigh 30g of peptone, 4g of beef extract, 4g of sodium chloride, 32g of lactose or glucose, and 20g of calcium carbonate;
[0042] ②Put the weighed peptone, beef extract, and sodium chloride in 500ml of pure water, heat to dissolve, adjust the pH to 7.0, and then replenish to 1500ml with pure water;
[0043] ③ Distribute the above-prepared medium into Φ15×150mm test tubes (9ml each), 250ml Erlenmeyer flasks (150ml each), and 1000ml Erlenmeyer flasks (750ml each), and add weighed lactose or Glucose, wrapped up and placed in a sterilizing pot, sterilized at 120°C for 25 minutes, cooled for later use.
[0044] 2) Preparation of 100L fermentation broth:
[0045]①Take a slant for production, transfer Streptococcus lactis into the first-level test tube at 39°C for 22 hours according to the operation requirements of the inclin...
Embodiment 3
[0053] Preparation of 150L third-stage Streptococcus lactis fermentation broth:
[0054] 1) Preparation of primary, secondary and tertiary medium:
[0055] ①Weigh 30g of peptone, 4g of beef extract, 3.5g of sodium chloride, 35g of lactose or glucose, and 10g of calcium carbonate;
[0056] ②Put the weighed peptone, beef extract, and sodium chloride in 500ml of pure water, heat to dissolve, adjust the pH to 6.5, and replenish to 1500ml with pure water;
[0057] ③ Distribute the above-prepared medium into Φ15×150mm test tubes (9ml each), 250ml Erlenmeyer flasks (150ml each), and 1000ml Erlenmeyer flasks (750ml each), and add weighed lactose or Glucose, wrapped up and placed in a sterilizing pot, sterilized at 115°C for 30 minutes, cooled for later use.
[0058] 2) Preparation of 150L fermentation broth:
[0059] ①Take a slant for production, transfer Streptococcus lactis into the first-level test tube at 37°C for 20 hours according to the operation requirements of the inclined...
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