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Method for purifying isomaltose hypgather by virtue of sequential simulated moving chromatography (SSMB)

A technology of isomaltose oligosaccharide and simulated mobile chromatography, which is applied in the fields of oligosaccharides, chemical instruments and methods, sugar derivatives, etc., can solve the problem of inability to efficiently purify isomaltose oligosaccharides, etc., so as to improve production efficiency and simplify operation steps. , the effect of reducing production loss

Active Publication Date: 2014-07-23
HEILONGJIANG HAOTIAN CORN DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem that isomaltooligosaccharide cannot be purified efficiently, and a method for purifying isomaltooligosaccharide by using sequential simulated mobile chromatography is studied

Method used

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  • Method for purifying isomaltose hypgather by virtue of sequential simulated moving chromatography (SSMB)
  • Method for purifying isomaltose hypgather by virtue of sequential simulated moving chromatography (SSMB)
  • Method for purifying isomaltose hypgather by virtue of sequential simulated moving chromatography (SSMB)

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Embodiment 1

[0028] The method for purifying isomaltooligosaccharides by sequential simulated mobile chromatography of the present invention uses sequential simulated mobile chromatography equipment for purification in the test, and the sequential simulated mobile chromatography equipment used in the present invention consists of 6 chromatographic columns (35mm in diameter and 1000mm in length) , each chromatographic column is 1L, a total of 6L, and the inlet and outlet are controlled by a solenoid valve. It is achieved through the following steps:

[0029] A) Deployment of feed syrup: Desalted and decolorized isomaltooligosaccharide syrup is prepared to a concentration of 60%;

[0030] B) Filtration: filter the syrup solution obtained in step A) with a micron filter to obtain a clear liquid without visible impurities;

[0031] C) Purification by sequential simulated mobile chromatography: the syrup obtained above is purified by sequential simulated mobile chromatography equipment. The s...

Embodiment 2

[0040] The method for purifying isomaltooligosaccharides by sequential simulated mobile chromatography of the present invention uses sequential simulated mobile chromatography equipment for purification in the test, and the sequential simulated mobile chromatography equipment used in the present invention consists of 6 chromatographic columns (35mm in diameter and 1000mm in length) , each chromatographic column is 1L, a total of 6L, and the inlet and outlet are controlled by a solenoid valve. It is achieved through the following steps:

[0041] A) Deployment of feed syrup: Desalted and decolorized isomaltooligosaccharide syrup is prepared to a concentration of 60%;

[0042] B) Filtration: filter the syrup solution obtained in step A) with a micron filter to obtain a clear liquid without visible impurities;

[0043] C) Purification by sequential simulated mobile chromatography: the syrup obtained above is purified by sequential simulated mobile chromatography equipment. The s...

Embodiment 3

[0052] The method for purifying isomaltooligosaccharides by sequential simulated mobile chromatography of the present invention uses sequential simulated mobile chromatography equipment for purification in the test, and the sequential simulated mobile chromatography equipment used in the present invention consists of 6 chromatographic columns (35mm in diameter and 1000mm in length) , each chromatographic column is 1L, a total of 6L, and the inlet and outlet are controlled by a solenoid valve. It is achieved through the following steps:

[0053] A) Deployment of feed syrup: Desalted and decolorized isomaltooligosaccharide syrup is prepared to a concentration of 60%;

[0054] B) Filtration: filter the syrup solution obtained in step a) with a micron filter to obtain a clear liquid without visible impurities;

[0055] C) Purification by sequential simulated mobile chromatography: the syrup obtained above is purified by sequential simulated mobile chromatography equipment. The s...

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Abstract

The invention relates to a method for purifying isomaltose hypgather by virtue of sequential simulated moving chromatography, wherein preprocessed isomaltose hypgather syrup is used as a raw material; isomaltose hypgather is purified by adopting sequential simulated moving chromatography equipment; deionized water is used as the eluent; the working temperature is at 50-70 DEG C; sodium type strong acidic cationic gel resin is used as working resin; isomaltose hypgather syrup with high purity is obtained; the higher yield is realized. The method disclosed by the invention is low in operation cost and production energy consumption, high in automation degree, continuous for production and high in production efficiency.

Description

technical field [0001] The invention belongs to the field of food industry, and specifically relates to a sequential simulated mobile chromatography separation and purification technology, in particular to a sequential simulated mobile chromatography purification technology for isomaltooligosaccharides. Background technique [0002] Isomaltooligosaccharide is an oligosaccharide with 2-8 glucose molecules linked by α-1,6 glycosidic bonds. Under normal circumstances, isomaltooligosaccharide is not fermented by yeast, and can effectively promote the growth and reproduction of beneficial bacteria in the human body - bifidobacteria, so it is also called "bifidobacteria growth-promoting factor". Bifidobacteria have many health functions , and isomaltooligosaccharide as a bifidobacterium-promoting factor has naturally attracted people's attention. At the same time, isomaltooligosaccharide has excellent processing characteristics, and its application in various foods has no special...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07H3/06C07H1/06
Inventor 李良玉李朝阳贾鹏禹吕获柱王学群张丽萍曹龙奎
Owner HEILONGJIANG HAOTIAN CORN DEV