Antigen and kit for detecting anti-human-papillomavirus antibody and application of antigen

A technology for anti-human papilloma and detection of human body, applied in the direction of virus/bacteriophage, virus, virus peptide, etc., can solve the problems of false negative, long detection time, and uneven sampling, so as to relieve pain and prevent cancer Effect

Inactive Publication Date: 2014-07-23
ATTOGEN BIOMEDICAL SUZHOU INC
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, HC2 and CareHPV detection methods use in vivo sampling, which is aggressive, expensive, and takes a long time to detect (at least 6 hours for HC2)
The PCR metho...

Method used

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  • Antigen and kit for detecting anti-human-papillomavirus antibody and application of antigen
  • Antigen and kit for detecting anti-human-papillomavirus antibody and application of antigen
  • Antigen and kit for detecting anti-human-papillomavirus antibody and application of antigen

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Embodiment Construction

[0026] In order to understand the technical content of the present invention more clearly, the following specific examples are enumerated in detail. However, the specific examples are for illustration only, not limitation of the present invention.

[0027] 1. Materials and methods

[0028] 1. Reagents and medicines

[0029] Horseradish peroxidase-labeled goat anti-human (Goat Anti-Human IgG, Fc) was purchased from PrieceProduct t#31413.

[0030] Sodium chloride, potassium chloride, disodium hydrogen phosphate, and potassium dihydrogen phosphate were purchased from Sinopharm Chemical Reagent Co., Ltd. Tween-20 was purchased from AMRESCO. Fetal bovine serum was purchased from Beijing Yuanheng Jinma Biotechnology Development Co., Ltd.

[0031] 2. Instruments and consumables

[0032] Electronic balance (Mettler-Toledo Instruments Shanghai Co., Ltd.); 85-1 constant temperature magnetic stirrer (Jintan Jerry Electric Co., Ltd., Jiangsu); MULTISKAN MK3 microplate reader (Thermo); ...

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Abstract

The invention relates to human papillomavirus (HPV) oncogene protein as an antiagen for detecting an anti-human-papillomavirus antibody, and the antigen has an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 3. The invention also provides an immunodetection kit which comprises the antigen for detecting anti-HPV antibody. The invention also provides application of the antigen used for detecting anti-HPV antibody to detect anti-HPV antibody in human blood or saliva. The antigen used for detecting anti-HPV antibody is capable of detecting an HPV antibody in blood or saliva with high sensitivity and high specificity, and thus the antigen helps to provide basis for doctors to accurately diagnose patient cervical intraepithelial neoplasia and cervical carcinoma transformed from HPV virus oncogene, and the immunodetection kit prepared by employing the antigen is capable of rapidly simply accurately detecting the PHV antibody in blood or saliva and is suitable for large-scale popularization and application.

Description

technical field [0001] The present invention relates to the technical field of antibody detection, in particular to the technical field of anti-human papillomavirus (Human Papillomavirus, HPV) antibody detection, and specifically refers to an antigen for detecting anti-human papillomavirus antibody and related immune detection kits and applications. It can be used for high-risk human papillomavirus (HPV) subtypes HPV16 and HPV18 that are prevalent in China. Background technique [0002] Cervical cancer is a common malignant tumor of the female reproductive system, ranking second among female malignant tumors. A large number of studies have found that HPV (Human Papillomavirus) is the culprit of cervical cancer and can also cause a variety of other tumors, including reproductive tract, breast, digestive tract, and respiratory tract cancers. In recent years, the spread of HPV in the Chinese population has increased unabated and tends to be younger, so it is very necessary for...

Claims

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/56983C07K14/005C12N2710/20022G01N33/57411
Inventor 常小迦张杰成
Owner ATTOGEN BIOMEDICAL SUZHOU INC
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