Method for producing biodiesel using microorganisms without drying process
A technology of biodiesel and microorganisms, which is applied to the preparation of biological raw materials, biofuels, and liquid hydrocarbon mixtures, and can solve problems such as expensive and complicated processes
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Embodiment 1
[0077] Method for producing biodiesel without drying and lipid extraction steps
[0078] Microalgae culture
[0079] To prepare FAME for use as biodiesel, microalgae Chlorella vulgaris AG10032 (provided by Biological Resource Center (BRC), Korea) was cultured in BG11 medium in a 7 L fermenter (see Rippka, R., DeReuelles, J., Waterbury, J.B., Herdman, M.&Stanier, R.Y.(1979).Generic assignments, strain histories and properties of pure cultures of cyanobacteria.J Gen Microbiol111, 1-61) were cultivated for 14 days, and the fermentor was cultured at 0.1 Air is replenished at a rate of v / v / m and passed by light at 120 μmol m -2 the s -1 irradiated. Measure dry cell weight of cultured microalgae. 50 mL of the cultured microalgae was centrifuged at 4000 rpm for 5 minutes at 25° C. using a 50 mL conical tube, and then the supernatant was removed to obtain a pellet (with a dry weight of about 0.1 g of wet biomass).
[0080] Transesterification reaction
[0081] 0.1 g of the pel...
Embodiment 2
[0099] is used to optimize the RSM (response surface method) analysis of transesterification
[0100] Add the precipitate to a predetermined amount of methanol, disperse evenly under stirring, and then use Minitab14 to carry out RSM to obtain in-situ transesterification efficiency according to the amount of catalyst (G.Vicente et al.: Industrial Crops and Products8 (1998) 29-35) (Fig. 4) .
[0101] As a result, the amount of FAME can be estimated from the amount of catalyst, the amount of biomass, and the reaction temperature. In addition, it was found that there was no significant difference in the amount of biodiesel produced in the temperature range of 4°C to 70°C, so the reaction can be efficiently performed at room temperature (ie, 25°C). Furthermore, increasing the amount of biomass was found to have no significant effect on the amount of FAME produced (Figures 4B and 4D). However, the amount of biodiesel showed a pattern with respect to the amount of catalyst, where ...
Embodiment 3
[0102] Discriminate transesterification according to the amount of catalyst by RSM analysis
[0103] In situ transesterification efficiency according to catalyst amount was measured by RSM using biomass dispersed in methanol.
[0104] Specifically, 0.1 g of the precipitate (wet biomass) obtained in Example was added to 100 mL of methanol and dispersed under stirring for 1 hour. The biomass uniformly dispersed in methanol was added to a 500mL double-jacketed reactor, and 0.00g, 0.01g, 0.02g, 0.05g, 0.10g, 0.20g, 0.50g, 1.00g, 2.00g and 3.00 g of NaOH was used as a catalyst and transesterification was carried out at 25° C. (room temperature) with stirring at 300 rpm. Furthermore, the type and amount of FAME produced after transesterification was identified.
[0105] As a result, similar to the RSM analysis results, the amount of catalyst and the amount of FAME produced were inversely proportional to each other. Similar amounts of FAME were produced when the amount of cataly...
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