Method for extracting and purifying nicotine by using polyethylene glycol (PEG)-disodium hydrogen phosphate aqueous two-phase system

Abstract

The invention discloses a method for extracting and purifying nicotine by using a polyethylene glycol (PEG)-disodium hydrogen phosphate aqueous two-phase system. The method comprises the steps of putting 2.8-3.2g of PEG2000 and 1.8-2.2g of disodium hydrogen phosphate into a container, adding 2-4ml of deionized water, shaking out, adding a nicotine solution to be extracted and purified, then adding deionized water again until the total weight reaches 8-12g, shaking out for 3-7 minutes, putting into a water bath kettle at 35-45 DEG C, standing, and separating nicotine in an upper phase after stably layering. The method disclosed by the invention is used for separating and purifying nicotine by adopting the PEG-disodium hydrogen phosphate aqueous two-phase system, and screening and optimizing the process conditions of aqueous two-phase extraction, has the advantages of easy phase splitting, cheap price, efficient and mild extraction, no toxicity, small activity loss of extracts and the like, and is a green process; meanwhile, an aqueous two phase disclosed by the invention has relatively strong selectivity to nicotine, is high in degree of enrichment, and has relatively strong practical values.

Description

technical field [0001] The invention relates to a method for extracting and purifying nicotine by using a polyethylene glycol-disodium hydrogen phosphate two-phase system. Background technique [0002] Nicotine, also known as nicotine, is mainly found in tobacco leaves. It is a natural alkaloid, which accounts for about 95% of the total tobacco alkaloids and 0.50-6.0% of the dry weight of tobacco. High-purity nicotine As an additive in the tobacco industry, it can improve the quality of cigarettes and make cigarette substitutes. In agriculture, nicotine comes from nature and has no residue. The solution containing nicotine sulfate can prevent and control crop pests such as aphids in field crop production. Nicotine series pesticides belong to plant insecticides, which have the characteristics of steam fumigation, stomach poisoning, contact killing and rapid degradation without residue. Green insecticides and biological pesticides meet the requirements of green food producti...

AI Technical Summary

Problems solved by technology

[0005] "Research and Development of Natural Products" 2012 12th issue, Liu Baoliang, Cao Guiping and Zhang Jintao published the title "Extraction of solanesol and Research on nicotine", which uses a new type of two-phase system as acetone-dipotassium hydrogen phosphate two-phase system to extract solanesol and nicotine. Although the research has made some breakthroughs, it also uses acetone, a toxic organic solvents, and has the defects of low extraction rate and little industrial value