Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A triple gold label detection test strip for antibodies against classical swine fever virus, porcine reproductive and respiratory syndrome virus, and pseudorabies virus

A porcine pseudorabies virus, respiratory disorder technology, applied in biological testing, measuring devices, material inspection products, etc., can solve -4 days and sometimes even more time, reduce detection costs, and there is no colloidal gold for simultaneous detection of disease antibodies. Detect problems such as test paper cards to achieve effective technical support and easy use

Active Publication Date: 2016-05-11
GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection mode of swine disease antibodies is mostly to collect serum samples from farms and then send them to special testing units for testing by ELISA method. It usually takes 3-4 days or even more time from sample submission to report results, which is not conducive to Reduce inspection cost and increase inspection speed
[0008] As far as the research and development of colloidal gold test strips is concerned, most of the current literature reports are single and multiple test strips for pathogen detection and single test strips for swine disease antibody detection. There is no simultaneous detection of CSFV, PRRSV, and PRV. Colloidal gold detection test paper card for antibodies against various diseases, and most of the existing gold-labeled antigens for detection use expressed proteins or whole virus antigens, and the use of peptides as gold-labeled antigens has not been reported yet.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A triple gold label detection test strip for antibodies against classical swine fever virus, porcine reproductive and respiratory syndrome virus, and pseudorabies virus
  • A triple gold label detection test strip for antibodies against classical swine fever virus, porcine reproductive and respiratory syndrome virus, and pseudorabies virus
  • A triple gold label detection test strip for antibodies against classical swine fever virus, porcine reproductive and respiratory syndrome virus, and pseudorabies virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Triple Gold Label Detection Test Strip for Antibodies of CSFV, PRRSV and PRV

[0050] 1. Screening of reactogenic peptides

[0051] Use biological software to compare the peptide sequences, analyze their antigenicity, and screen to obtain the following specific peptide antigens, which will be sent to the biological company for synthesis.

[0052] CSFV-specific reactogenic polypeptide:

[0053] SEQ ID NO.1: c-HKVRNEVMVHWFDDE (CysHisLysValArgAsnGluValMetValHisTrpPheAspAspGlu)

[0054] SEQ ID NO.2: GLSTAENALLVALFGYV (GlyLeuSerThrAlaGluAsnLeuLeuValAlaLeuPheGlyTyrVal)

[0055] SEQ ID NO.3: CTAVSPTTLRTE (CysThrAlaValSerProThrThrLeuArgThrGlu)

[0056] SEQ ID NO.4: KYNTTLLNGSA-c (LysTyrAsnThrThrLeuLeuAsnGlySerAlaCys)

[0057] Porcine reproductive and respiratory syndrome virus specific reactogenic polypeptide:

[0058] SEQ ID NO.5: c-GFMVPGLSSEGHLT (Cys-GlyPheMetValProProGlyLeuSerSerGlyHisLeuThr)

[0059] SEQ ID NO.6: KTKSVKSLPGNKPVP-c (LysThrLysSerValLysSerLeuP...

Embodiment 2

[0097] Example 2 Performance Verification of CSFV, PRRSV, and PRV Antibody Triple Gold Label Detection Test Strips

[0098] Prepare 10 kinds of colloidal gold detection test paper cards with SEQIDNO.1--SEQIDNO.10 respectively. Detect CSFV, PRRSV, and PRV clinical serum samples with IDEXX swine disease antibody ELISA kit, select 60 corresponding positive samples and 60 negative samples, and prepare them as standard negative and positive serum for judging the sensitivity of the test strip; use ELISA kit for detection Confirm the positive and negative sera of CSFV, PRRSV, PRV, PCV2 (circovirus type 2), PPV (parvovirus), FMDV (foot-and-mouth disease virus) etc. as the quality control serum specific for the test strip card, and detect SEQ ID NO.1-SEQ ID NO. 10 The sensitivity and specificity of the polypeptide, the characteristics of the polypeptide of SEQ ID NO.1-SEQ ID NO.10 are judged according to the color development result.

[0099] Table 3 Sensitivity test of classical swin...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a triple gold label detection test strip for swine fever virus, porcine reproductive and respiratory syndrome virus and pseudorabies virus antibody, which comprises a sample pad, a gold standard pad, a nitrocellulose membrane and absorbent paper. Gold-labeled CSFV reactogenic polypeptides, gold-labeled PRRSV reactogenic polypeptides, and gold-labeled pig PRV reactogenic polypeptides are sprayed on the gold-labeled pad; three detection lines and one quality control line are provided on the nitrocellulose membrane, so CSFV reactogenic polypeptides, PRRSV reactogenic polypeptides, and PRV reactogenic polypeptides are immobilized on the three detection lines respectively, and CSFV antibodies, PRRSV antibodies and PRV antibodies are immobilized on the quality inspection lines. The test strip of the present invention has the advantages of multiple, specific, sensitive, easy to use, fast, etc. It can provide efficient and practical antibody detection technology and products for the grassroots of pig breeding in my country, and provide effective technical support for the supervision of epidemic prevention of pig epidemics.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a triple gold label detection test strip for antibodies to swine fever virus, porcine reproductive and respiratory syndrome virus and pseudorabies virus prepared by using polypeptide antigens. Background technique [0002] In recent years, the main diseases that have harmed my country's pig industry include porcine reproductive and respiratory syndrome, swine fever and pseudorabies, foot-and-mouth disease and ring, among which blue ear disease is the most serious. Developed countries in the pig industry in the United States and Europe generally adopt a disease purification strategy to prevent and control some important diseases such as foot-and-mouth disease, swine fever, and pseudorabies, which have stopped using vaccines, and take strict quarantine, monitoring, and purification measures. Due to the existing basic conditions and the epidemic situation in...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68G01N33/569G01N33/558
CPCG01N33/56983G01N33/588G01N33/68G01N2333/032G01N2333/183
Inventor 林礼锟李其昌陈善真王志花刘博奇陈克宏王贵平
Owner GUANGDONG HAID ANIMAL HUSBANDRY & VETERINARY RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com