Peach ssap molecular marker primer combination, molecular marker combination and its application in genetic diversity analysis of peach varieties

Abstract

The invention discloses a peach SSAP (Source Service Access Point) molecular marker primer combination, a peach SSAP molecular marker combination and an application of the peach SSAP molecular marker combination in analysis on the genetic diversity of peach varieties, wherein the peach SSAP molecular marker primer combination comprises an LTR (Long Terminal Repeat) primer, a selective amplification primer and a tail primer; the peach SSAP molecular marker combination comprises ten molecular markers JY01, JY02, JY03, JY04, JY05, JY06, JY07, JY08, JY09 and JY10. Due to the design of a peach retrotransposon LTR sequence primer, a selective amplification product is proved to be clear and abundant in amplification strip and have high efficiency, reliability and practicability through fluorescent capillary electrophoresis detection; in addition, a selective amplification PCR (Polymerase Chain Reaction) system is optimized, and a tail sequence is added, so that the traditional selective amplification PCR system is improved, and the cost for carrying out relevant researches by using the molecular markers is reduced. The SSAP molecular marker combination disclosed by the invention has relatively high polymorphism in the plurality of peach varieties, comprises stably existing markers and can be used for peach variety identification and genetic diversity analysis.

Description

technical field [0001] The invention relates to a primer combination of peach SSAP molecular markers, a combination of peach SSAP molecular markers and the application thereof in the analysis of genetic diversity of peach varieties, belonging to the field of molecular biology. Background technique [0002] Peach [Prunus persica (L.) Batsch] belongs to Rosaceae (Rosaceae), Prunus (Prunus L.), rich in germplasm resources, originated in western China. According to statistics, my country has the most abundant peach germplasm resources in the world, and various varieties and excellent lines have been accumulated through natural variation and artificial breeding. There are more than 1,600 peach germplasm resources preserved by the Zhengzhou Fruit Tree Research Institute of the Academy of Agricultural Sciences and the Horticultural Research Institute of the Jiangsu Academy of Agricultural Sciences. [0003] A large number of studies have shown that my country's peach germplasm reso...

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Problems solved by technology

[0005] At present, SSAP molecular marker technology has been successfully used in the genetic diversity analysis of grape, barley, apple and other species (Labra, 2004; Queen, 2004; Venturi, 2006), but the development of retrotransposon primers is race-specific, and different species There is great heterogeneity among retrotransposon primers, so far there is no report on the application of SSAP molecular marker technology in the analysis of genetic diversity in peach cultivars

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