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Method for detecting Hg<2+> with signal-off chemiluminescence method

A chemiluminescence method and solution technology, which is applied in the fields of analytical chemistry and optical DNA biosensing, can solve the problems of long DNA sensor preparation cycle, short electrode storage time, cumbersome electrode preparation process, etc., and achieve short experimental cycle and fast response speed , low cost effect

Inactive Publication Date: 2014-08-06
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, DNA sensors have disadvantages such as long preparation period, cumbersome electrode preparation process, and short electrode storage time. The limitations of many factors force researchers to seek new detection methods.

Method used

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  • Method for detecting Hg&lt;2+&gt; with signal-off chemiluminescence method
  • Method for detecting Hg&lt;2+&gt; with signal-off chemiluminescence method
  • Method for detecting Hg&lt;2+&gt; with signal-off chemiluminescence method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1) 20mM Tris-NaClO with pH=9 4 Dissolve the solid G-DNA in the buffer solution, mix it with a vortex shaker, and then add a certain concentration of K to the above system + And hemin solution, put it for 1-2h to form a G-DNA structure with peroxidase-like activity;

Embodiment 2

[0028] Control assay system G-DNA concentration is 100nM, K + Concentration is 10mM, hemin concentration is 200nM, H 2 o 2 The concentration is 10mM, the concentration of luminol is 500μM, and the determination of adding different concentrations of Hg 2+ (10 -11 mol / L, 10 -10 mol / L, 10 -9 mol / L, 10 -8 mol / L, 10 -7 mol / L and 10 -6 mol / L) system chemiluminescence intensity. The change (ΔI) of chemiluminescence intensity (Intensity) is shown in Table 1.

[0029] Table 1 with different concentrations of Hg 2+ Intensity of chemiluminescence after action

[0030]

Embodiment 3

[0032] Control assay system G-DNA concentration is 100nM, K + Concentration is 10mM, hemin concentration is 200nM, H 2 o 2 The concentration was 10 mM, the luminol concentration was 500 μM, the pH of the solution was changed, and the chemiluminescence intensity of the system was measured. The chemiluminescence intensity is shown in Table 2.

[0033] Table 2 with different concentrations of Hg 2+ Intensity of chemiluminescence after action

[0034]

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Abstract

The invention belongs to the technical field of analytical chemistry and optical DNA biosensing and relates to a method for detecting Hg<2+> with a signal-off chemiluminescence method. The method comprises the following steps: a mispairing hairpin DNA sequence containing a G-DNA structure fragment and part of T-T is adopted; when K<+> and hemin exist, the hairpin DNA configuration is changed to form a quadruplex G-DNA structure with peroxidase catalytic activity, and the structure can catalyze hydrogen peroxide oxidation luminol to generate chemiluminiscence; when Hg<2+> exists in a system, as the hairpin DNA structure is stabilized through the formation of a T-Hg-T structure, the quadruplex G-DNA structure cannot be formed, and chemiluminiscence is quenched; according to the influence of the change of concentrationof Hg<2+> on the quenching degree of chemiluminiscence, the detection on Hg<2+> is realized. The optical DNA biosensing method has the advantages of easiness in operation, short response time, good stability, high flexibility and high selectivity.

Description

technical field [0001] The invention belongs to the technical field of analytical chemistry and optical DNA biosensing, and relates to the detection of Hg by using a "signal-off" type G-DNA enzyme catalyzed chemiluminescence method. 2+ detection method, this method can quickly and effectively realize the detection of Hg in water 2+ detection. Background technique [0002] Heavy metals are a class of inorganic pollutants that are difficult to degrade in the environment, widely exist in the atmosphere, water, soil and other environmental media and cause serious environmental pollution. Heavy metals in the environment have strong bioaccumulation, can enter the human body through various channels and can accumulate in the body for a long time. When the concentration exceeds a certain limit, it will affect various organs (liver, kidney, etc.) and systems (urinary System, nervous system, etc.) cause serious damage, thus causing great harm to the human body, especially the highly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
Inventor 刘新会梁刚刘冠男巩文雯成登苗侯静梁宝翠
Owner BEIJING NORMAL UNIVERSITY
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