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Beauveria bassiana for preventing radysia odoriphaga and application thereof

A technology of Beauveria bassiana and strains, applied in the field of biological control, can solve the problems of pesticide residue, excess, complicated operation and the like, and achieve the effects of simple production method, simple use method and good insecticidal effect.

Active Publication Date: 2014-08-13
INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the shortcomings of physical control, such as cumbersome operation and great influence of the environment, chemical methods such as pesticide root irrigation are mainly used for control in production. Excessive or even illegal use of prohibited and restricted highly toxic pesticides for prevention and control, causing environmental pollution, pesticide residues, and endangering human health problems

Method used

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  • Beauveria bassiana for preventing radysia odoriphaga and application thereof
  • Beauveria bassiana for preventing radysia odoriphaga and application thereof
  • Beauveria bassiana for preventing radysia odoriphaga and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Embodiment 1, the preparation of Beauveria bassiana bacterial strain Lu71 spore suspension

[0028] Proceed as follows:

[0029]Beauveria bassiana strain Lu71 (preserved on May 26, 2014 in the General Microbiology Center of China Committee for the Collection of Microorganisms, address: No. 3, Courtyard No. 1, Beichen West Road, Chaoyang District, Beijing, Chinese Academy of Sciences Institute of Microbiology, preservation number is CGMCC No.9252) inoculated on SDAY plate medium (composition and weight ratio of SDAY plate medium are: peptone 10g, yeast 10g, glucose 40g, agar 15g, distilled water 1000mL, natural pH) cultured at 25±1°C under constant temperature and light (14L:10D) for 10 days until sporulation. Use an inoculation loop to scrape conidia into 0.05% (v / v) sterile Tween 80 (analytical pure biochemical reagent) aqueous solution by volume percentage, stir with a magnetic stirrer for 30 minutes to obtain a pure spore suspension, and pass through a hemocytometer...

Embodiment 2

[0030] Embodiment 2: Insecticidal efficacy test of Beauveria bassiana strain Lu71 of the present invention to tardy-eyed fungus

[0031] Pour the sterilized plate into 3 mm of sterilized aqueous solution containing 1.5% agar, cover with double-layer filter paper for moisture retention, cut the leeks into 10 mm leek segments, soak them in the concentration prepared in Example 1, respectively 1 × 10 5 , 1×10 6 , 1×10 7 , 1×10 8 , 5×10 8 spores / mL of Lu71 spore suspension for 10 min, taken out and placed in the above-mentioned plate, inoculated with healthy and lively second-instar larvae of T. spp. L:D) under the condition of feeding, fresh leek leaves were fed to the larvae of T. For each treatment of 30 larvae of Bradyphagia leeks, 3 repetitions were set up, and the treatment with 0.05% (V / V) Tween-80 aqueous suspension was used as a control. The natural mortality rate of the control was within 5% and no dead worms were considered effective. experiment. After pesticide a...

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Abstract

The invention relates to a beauveria bassiana strain Lu71 for preventing radysia odoriphaga and belongs to the field of biological prevention and control. The strain was preserved in China General Microbiological Culture Collection Center on May 26, 2014 with the preservation number of CGMCC No.9252. The invention further discloses an insecticide containing the beauveria bassiana strain Lu7. The beauveria bassiana strain Lu7 provided by the invention is high in toxicity to beauveria bassiana, strong in specificity and good in insecticidal efficacy. In addition, the strain provided by the invention belongs to the field of biological prevention and control, does not cause environmental pollution and is harmless to other natural enemies. Furthermore, the use of pesticides can be replaced or reduced and the problem of environmental pollution can be avoided.

Description

technical field [0001] The invention belongs to the technical field of biological control, and in particular relates to a Beauveria bassiana used for preventing and treating the tardy-eyed fungus and the application of the Beauveria bassiana for preventing and treating the tardy-eyed fungus. Background technique [0002] Bradysia odoriphaga, also known as chive maggot, belongs to Diptera, Odoriphagae, is one of the main pests of green onions and garlic vegetables, and it is distributed all over the country. Chive tardigrades damage the bulbs and tender stems of chives with their larvae. The newly hatched larvae first damage the base of the leek sheath and the upper end of the bulb. In spring and autumn, the young stems of leeks are mainly caused to rot, causing the leaves of leeks to wither and die. In summer, the larvae move downward and bore into the bulbs. In severe cases, the bulbs rot and the whole pile of leeks dies. There are physical control (sweet and sour liquid...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P7/04C12R1/645
Inventor 宋健曹伟平杜立新王金耀冯书亮
Owner INST OF PLANT PROTECTION HEBEI ACAD OF AGRI & FORESTRY SCI