Method for preparing water-soluble active polysaccharide in Calvatia candida mycelia

Abstract

The invention discloses a method for preparing a water-soluble polysaccharide in Calvatia candida mycelia. The method comprises the steps of incubating Calvatia candida bacteria on a solid slant culture medium and culturing at a constant temperature to obtain slant seeds; inoculating the cultured slant seeds into a primary liquid seed culture medium and culturing; inoculating the cultured primary liquid seeds into a secondary liquid seed culture medium and culturing; inoculating the cultured secondary liquid seeds and fermenting; separating mycelia from the fermentation liquid, washing with clean water, drying and grinding to obtain dried mycelia powder; extracting dried mycelia powder or carrying on ultrasonic extraction, concentrating liquid, precipitating with ethanol and collecting to obtain the water-soluble active Calvatia candida crude polysaccharide precipitate, dissolving the crude polysaccharide precipitate in hot water to remove the free proteins and small molecule impurities, concentrating, precipitating with ethanol, and drying to obtain a Calvatia candida water-soluble active polysaccharide purified extract. The method disclosed by the invention is feasible and efficient and has the advantages of simple preparation operation, low temperature and short time.

Description

[0001] technical field [0002] The invention belongs to the field of bioengineering and technology, and more specifically relates to a method for preparing water-soluble active polysaccharides in mycelia of alba bald puffballs. Background technique [0003] White bald puffball, also known as white puffball in Chinese, is a medicinal fungus of Basidiomycetes, cinerea, cinerea, and puffball genus. It is one of the famous medicinal fungi in my country. It is mainly distributed in Hebei, Shanxi, Liaoning, Heilongjiang, Shaanxi, Gansu, Xinjiang and other regions. The fruiting bodies are small, oblate, subspherical, pear-shaped, light brown-gray, and have well-developed root-like hyphae. The outer capsule is thin, powdery, and streaked, and the inner capsule is firm and brittle. Spores are honey-yellow to light brown, spores are spherical, light blue-yellow, 4-5 μm in diameter. Smooth or very fine warts, often with small stalks. Sporocysts of the same color, rare septa, unifo...

AI Technical Summary

Problems solved by technology

However, the traditional production method of Baimabo is solid cultivation. This method has a long cycle, a large area, and is limited by seasons and raw materials, so that the yield of active polysaccharides in fruiting bodies is low and difficult to promote, so that it cannot be fully harvested. use

Extracting water-soluble active polysaccharides from the white bald puffball mycelium is still not reported in the literature. Other puffball polysaccharides are extracted from the fruiting bodies, extracted in hot water, deproteinized by the Sevag method, and prepared by ethanol precipitation. The method has problems such as low extraction efficiency and long time