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Test strip for screening specific injurious insect allergens IgE (Immunoglobulin E) and preparation method thereof

A technology for test strips and allergens, which is used in measuring devices, instruments, disease diagnosis, etc., can solve the problems of cumbersome operation, poor sensitivity and precision, and long test time, and achieves simple operation, accurate results and low cost. Effect

Active Publication Date: 2014-08-20
BIOSINO BIO TECH & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are disadvantages of cumbersome operation, poor sensitivity and precision, and long test time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0028] Example Test strips for IgE screening of specific pest allergens and preparation methods thereof

[0029] The preparation of test strips for IgE screening of specific cockroach allergens includes the preparation of the antigen solution to be labeled, the preparation of the colloidal gold solution to be labeled, the colloidal gold labeled cockroach antigen, the purification of the colloidal gold cockroach antigen, and the purification of the colloidal gold protein particles. Particle size identification and coating of test strips. Specific steps are as follows:

[0030] 1. Preparation of antigen solution to be labeled

[0031] The preparation method of cockroach antigen is as follows: take 35g of cockroaches, add 200mL of water, after freezing, pulverize with a pulverizer, then filter with a cellulose acetate membrane with a pore size of 0.4μm, collect the cockroach powder on the filter membrane, add 200mL of toluene after drying to analyze Pure degreasing treatment, r...

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Abstract

The invention provides a test strip for screening specific injurious insect allergens IgE (Immunoglobulin E). The test strip is coated with a pest antigen marked by colloidal gold. The test strip utilizes a colloidal gold marking technology; firstly, the colloidal gold mark is used for marking the specific injurious insect allergens; then the marked allergens are uniformly sprayed on a cellulose acetate membrane or a nitrocellulose membrane to prepare a test strip product to carry out allergen detection; the test strip is simple and convenient to operate, accurate in result and low in cost and can realize multi-term free detection.

Description

technical field [0001] The invention relates to the technical field of colloidal gold labeling, in particular to a test strip for IgE screening of specific pest allergens and a preparation method thereof. Background technique [0002] Allergic disease is the most common disease in contemporary society. It is caused by widespread allergens. About 5-10% of the population in my country and 5-25% of the population in the United States and Europe have suffered from allergic infestation. Among them, young children And teenagers are particularly obvious, and harm is more serious. Allergic reaction (allergy) is a pathological immune response that causes physiological dysfunction and tissue damage when the immune body is exposed to the same allergen again. Involves various clinical departments, such as internal medicine including bronchial asthma, anaphylactic shock, serum sickness, connective tissue disease, glomerulonephritis, migraine, etc., surgery including transplant rejection,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/531G01N33/532
CPCG01N33/531G01N33/532G01N33/558G01N2800/24
Inventor 蒋琳
Owner BIOSINO BIO TECH & SCI
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