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A kind of citrus biological composite anti-mildew preservative and its preparation and use method

An anti-mildew preservative and citrus technology, applied in the field of citrus preservation, can solve the problems of allergies, poisoning, high toxicity, etc., and achieve the effects of reducing the mildew rate, enhancing the resistance to stress, and reducing losses

Active Publication Date: 2016-01-20
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional chemical anti-mildew preservatives are highly toxic and can easily cause people's allergies and poisoning, while the new biological preservatives are only proteins, polypeptides, certain lipids and organic acids, which have no or very toxic side effects on the human body. Small

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Adopt following mass, volume part raw material to prepare citrus biological composite anti-mold antistaling agent of the present invention: 1 part of glacial acetic acid, 2 parts of chitosan, Bacillus amyloliquefaciens ( Bacillus amylolique faciens ) 1.5 parts of HRH.317 fermentation broth, 1.5 parts of Tween-80, 0.3 parts of NaCl, and 93 parts of distilled water.

[0034] The preparation method of Bacillus amyloliquefaciens HRH.317 fermented liquid is:

[0035] (1) Preparation of liquid culture medium Add 0.5 part of beef extract, 1 part of peptone and 0.5 part of NaCl to 100 parts of distilled water, adjust the pH to 7.2, sterilize at 121 ° C for 20 minutes, and make a culture medium;

[0036] (2) Activation of Bacillus amyloliquefaciens HRH317 Under aseptic conditions, inoculate Bacillus amyloliquefaciens HRH.317 in the culture solution prepared in (1) with an inoculation loop, at 37°C, the rotating speed is 150r / min Vibrating culture in a constant temperature shake...

Embodiment 2

[0044] Adopt following quality, volume part raw material to prepare citrus biological compound anti-mold antistaling agent of the present invention: 1 part of glacial acetic acid, 3 parts of chitosan, bacillus amyloliquefaciens ( Bacillus amylolique faciens ) 2 parts of HRH.317 fermentation broth, 2 parts of Tween-802 parts, 0.5 parts of NaCl, 93 parts of distilled water.

[0045] The preparation method of Bacillus amyloliquefaciens HRH.317 fermentation broth is the same as that in Example 1.

[0046] The preparation method of citrus biocomposite anti-mildew preservative is: add 1 part (V / V) of glacial acetic acid in 93 parts of distilled water to make acetic acid solution; add 3 parts (W / V) of chitosan respectively in the acetic acid solution , 2 parts (V / V) of Bacillus amyloliquefaciens HRH317 fermentation broth, 2 parts (W / V) of Tween-80 and 0.5 part (W / V) of NaCl, and stir evenly.

[0047] The using method of citrus biological composite anti-mold antistaling agent is the ...

Embodiment 3

[0049] Adopt following quality, volume part raw material to prepare citrus biological composite anti-mold antistaling agent of the present invention: 1 part of glacial acetic acid, 2.5 parts of chitosan, Bacillus amyloliquefaciens ( Bacillus amylolique faciens ) 1.8 parts of HRH.317 fermentation broth, 1.7 parts of Tween-80, 0.4 parts of NaCl, and 93 parts of distilled water.

[0050] The preparation method of Bacillus amyloliquefaciens HRH.317 fermentation broth is the same as that in Example 1.

[0051] The preparation method of citrus biological composite anti-mildew preservative is: add 1 part (V / V) of glacial acetic acid in 93 parts of distilled water to make acetic acid solution; add 2.5 parts (W / V) of chitosan respectively in the acetic acid solution , 1.8 (V / V) Bacillus amyloliquefaciens HRH317 fermentation broth, 1.7 parts (W / V) of Tween-80 and 0.4 parts (W / V) of NaCl, and stir evenly.

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PUM

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Abstract

The citrus biological compound anti-mildew preservative of the present invention is composed of the following raw materials in parts by mass and volume: 1 part of glacial acetic acid, 2-3 parts of chitosan, 1.5-2 parts of Bacillus amyloliquefaciens HRH.317 fermentation broth, Tween-80 ?1.5-2 parts, NaCl? 0.3-0.5 parts, 93 parts of distilled water. The preparation method of the preservative is: first add glacial acetic acid to distilled water to make an acetic acid solution; then add chitosan, Bacillus amyloliquefaciens HRH317 fermented liquid, Tween-80 and NaCl to the acetic acid solution, and stir evenly. . The method of using this preservative is: wash the citrus without damage and disease with tap water and then immerse it in 0.1% sodium hypochlorite solution for disinfection for 1-2 minutes. After taking it out, wash the sodium hypochlorite with sterile water and dry it. Soak in the preservative for 30-40 seconds, take out and dry completely, then pack them in PE fresh-keeping bags, put 25-40 fruits in each bag, put them in plastic baskets, and store them in a room temperature of 18-23°C and a relative humidity of 70%- Store under the condition of 80% humidity or store at a storage temperature of 4-6°C and a relative humidity of 70%-80%.

Description

technical field [0001] The invention relates to the technical field of citrus preservation, in particular to the preservation of citrus by adopting a biological anti-mold antistaling agent and a method for preparing and using the anti-mold antistaling agent. Background technique [0002] Fruits and vegetables are one of the main foods for human beings, but they are prone to spoilage due to regional and seasonal influences. The spoilage of fruits and vegetables will cause unnecessary money loss and reduce the quality of fruits and vegetables. Among them, food-borne pathogens can produce a large amount of toxins, causing people to suffer from diseases such as digestive tract diseases, immune diseases and inflammation. Traditional chemical anti-mildew preservatives are highly toxic and can easily cause human allergies and poisoning, while the new biological preservatives are only proteins, polypeptides, certain lipids and organic acids, etc., which have no or very toxic side ef...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23B7/155
Inventor 郝林杨宇郝利平
Owner SHANXI AGRI UNIV
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