Preparation method for fluorescent molecular imprinted probe

Abstract

The invention discloses a preparation method for a fluorescent molecular imprinted probe. Cadmium telluride quantum dots are taken as a fluorescent element, and serve as a carrier after being subjected to silicon coating treatment, template molecules are replaced by phenyl phosphonic acid, and surface imprinting and antigen determinant methods are used for synthesizing the molecular imprinting probe. The method comprises the following steps of preparing a cadmium telluride quantum dot solution; preparing quantum dot silicon-coated microspheres; preparing a composite material taking the quantum dot silicon-coated microspheres as a core and a molecular imprinted layer as a shell; and preparing the fluorescent molecular imprinting probe. The method has the advantages that high sensitivity of the quantum dots and high selectivity of molecular imprinting are combined by the fluorescent molecular imprinted polymer, the surface of the carrier is modified by the molecular imprinted layer, and recognition sites are close to the surface, so that target molecules can be easily and selectively recognized; the fluorescent probe synthesized by the antigen determinant method can be used for recognizing the target molecules tyrosine phosphorylated peptide, and fluorescence enhancement effects are achieved.

Description

Technical field [0001] The present invention involves the preparation technology of the quantum dot fluorescent probe, especially the preparation method of a fluorescent molecular mark probe. Background technique [0002] Molecular printing technology is the technology of the molecular seal polymer that is fully matched with the target molecules on the shape, size, space structure and binding site.1993, 361 (6413): 645-647; L. X. Chen, S. F. XU, J. H. Li, Chem. Soc. Rev., 2011, 40 (5), 2922-2942.Molecular printing technology, as a separation technology, has three characteristics: predetermined, recognition, and practicality.At present, the molecular marks polymer has been successfully applied to the identification and analysis of small molecules, but the molecular seal polymer's identification of macroblags such as polypeptides or protein is still a severe challenge.Molecular weight, certain solubility, and complex structural changes.Despite various difficulties, methods such as ...

AI Technical Summary

Problems solved by technology

Ideal fluorescent probes have high sensitivity and must be able to specifically bind to the corresponding target analytes. Due to the high price of suitable natural antibodies, cumbersome extraction process, and poor stability, the advantages of quantum dots and molecular The combination of the characteristics of imprinting technology is of great significance

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