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Liquid fermentation medium for culturing edible mushroom liquid microbial strains and liquid microbial strain preparation method

A liquid strain and liquid fermentation technology, applied in the field of bioengineering, can solve the problems of low strain vigor, high pollution rate, production chaos, etc., and achieve the effects of reducing pollution rate, reducing labor costs, and saving seed production time

Inactive Publication Date: 2014-09-10
HEBEI LYUDI JIULE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, in the production, inoculation and cultivation of edible fungus liquid strains, there are problems such as low strain vigor, high pollution rate, and production confusion.

Method used

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  • Liquid fermentation medium for culturing edible mushroom liquid microbial strains and liquid microbial strain preparation method
  • Liquid fermentation medium for culturing edible mushroom liquid microbial strains and liquid microbial strain preparation method
  • Liquid fermentation medium for culturing edible mushroom liquid microbial strains and liquid microbial strain preparation method

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preparation example Construction

[0031] A method for preparing edible fungus liquid strains, comprising the following steps:

[0032] 1) Activation of slant strains: put the slant strains on PDA medium, and cultivate them at a constant temperature of 25°C for 7 days, and use them as primary strains;

[0033] 2) Cultivation of first-level liquid strains: put the seed liquid culture medium into a 300ml Erlenmeyer flask, the filling capacity of each bottle is 70-90ml, sterilize the seed liquid culture medium and inoculate the obtained in step 1 under a sterile environment The first-class strains were cultured in a shaker for 7 days;

[0034] 3) liquid fermentation: put the fermented liquid medium according to claim 1 into a fermenter, inoculate the liquid strain of step 2 gained in a sterile environment after the liquid medium is sterilized, and the inoculation amount is calculated as 8-12% of the culture medium, cultured with stirring for 3 days to make mycelium.

[0035] Production of liquid strains and its ...

Embodiment 1

[0039] A preparation method of Bailing mushroom liquid spawn, comprising the following steps:

[0040] 1) Activation of slant strains: put the slant strains on PDA medium, and cultivate them at a constant temperature of 25°C for 7 days, and use them as primary strains;

[0041] 2) Cultivation of the first-level liquid strain: put the seed liquid culture medium into a 300ml Erlenmeyer flask, the filling capacity of each bottle is 80ml, sterilize the seed liquid culture medium and inoculate the first-level obtained in step 1 under a sterile environment The strain, the inoculum amount is 8-12% of the culture medium by weight percentage, and it is placed in a shaker for 7 days; the seed liquid culture medium is: 3% corn flour, 2% wheat bran, 2% glucose, protein Chen 0.5%, MgSO 4 ·7H 2 O0.1%, KH 2 PO 4 0.1%, VB 1 10mg / L, soybean oil 0.03%. The initial pH value is 5.5, and the rotation speed of the shaker is 210r / min.

[0042] 3) liquid fermentation: put the liquid fermentati...

Embodiment 2

[0045] A preparation method of Bailing mushroom liquid spawn, comprising the following steps:

[0046] 1) Activation of slant strains: put the slant strains on PDA medium, and cultivate them at a constant temperature of 25°C for 7 days, and use them as primary strains;

[0047] 2) Cultivation of the first-level liquid strain: put the seed liquid culture medium into a 300ml Erlenmeyer flask, the capacity of each bottle is 70ml, sterilize the seed liquid culture medium and inoculate the first-level obtained in step 1 under a sterile environment Bacteria, the inoculation amount is 12% of the culture medium by weight percentage, and it is placed in a shaker for 7 days; the seed liquid culture medium is: 3% corn flour, 2% wheat bran, 2% glucose, and 0.5% egg white. %, MgSO 4 ·7H 2 O0.1%, KH 2 PO 4 0.1%, VB 1 10mg / L, soybean oil 0.03%. The initial pH value is 6.0, and the rotation speed of the shaker is 200r / min.

[0048] 3) liquid fermentation: put the liquid fermentation me...

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Abstract

The invention provides a liquid fermentation medium for culturing edible mushroom liquid microbial strains. The liquid fermentation medium for culturing the edible mushroom liquid microbial strains comprises, by weight, 1.5% of glucose, 3% of corn flour, 0.2% of peptone, 2% of soybean meal, 0.1% of MgSO4 7H2O, 40.1% of KH2PO, 10.01% of VB, 0.03% of soybean oil and the balance water. The invention further provides an edible mushroom liquid microbial strain preparation method. The preparation method includes activation of inclining microbial strains, cultivation of first-level liquid microbial strains and liquid fermentation. According to the edible mushroom liquid microbial strain production method, through the schemes such as liquid medium formulation optimization and fermentation condition optimization, the yield of cultivation of the liquid microbial strains can be increased by 32.8% compared with that of bagging cultivation of solid microbial strains, the contamination rate is reduced by 4%, the seed production time is shortened, and the labor cost is reduced.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a liquid fermentation medium for cultivating liquid strains of edible fungi and a preparation method of the liquid strains. Background technique [0002] With the increasing demand for edible fungi products in domestic and foreign markets, China's edible fungus industry has grown at an average annual rate of 17%. The production mode of edible fungus is gradually developing from the traditional family workshop operation and seasonal production mode to facility-based, modernized, annualized and large-scale production. Changes in production methods have driven the development of new technologies, new processes, and new equipment for edible fungi. In recent years, the use of liquid submerged culture technology to prepare liquid strains of edible fungi for production has become a research and development hotspot. Bacteria reports. The general promotion and application of liq...

Claims

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Application Information

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IPC IPC(8): A01G1/04C05G1/00
Inventor 李俊九
Owner HEBEI LYUDI JIULE BIOTECH
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