Polysaccharide of Prunus chinensis, extraction and purification method and its application as tobacco moisturizing agent
A technology of polysaccharide and moisturizing agent of P. chinensis, which is applied in the directions of tobacco, application, and treatment of tobacco, and achieves the effects of obvious moisturizing effect, cheap and easy-to-obtain reagents, and broad prospects for popularization and application.
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Embodiment 1
[0017] A method for extracting and purifying polysaccharides from Desmodium desmodium, comprising the following steps: extracting 300 g of dried desmodium desmodium with 1800 ml of ether Soxhlet for 2 hours to remove oil, and evaporating the residue to dryness. The residue after evaporating to dryness was extracted three times with 750ml boiling water, combined the boiling water extracts obtained three times, concentrated, centrifuged, and the supernatant was taken, and the supernatant was added with absolute ethanol 3 times the volume of the clear liquid for alcohol precipitation, and stood overnight (12h), centrifuge again to collect the precipitate. The precipitate was washed three times with 300ml ethanol and 300ml acetone in turn, and then dissolved in distilled water, then papain with a weight of 1‰ of the precipitate was added and placed in a water bath at 50°C for 2 hours, and the protein was removed by the Sevage method. Add 8g of activated carbon and place it in a 70...
Embodiment 2
[0020] A method for extracting and purifying polysaccharides from Desmodium desmodium, comprising the following steps: extracting 300g of dried Desmodium desmodium with 2000ml of ether Soxhlet for 4 hours to remove oil, and evaporating the residue to dryness. The residue after evaporating to dryness was extracted with 750ml of boiling water for 3 times, and the boiling water extracts obtained three times were combined, concentrated, centrifuged, and the supernatant was taken, and anhydrous ethanol with 3 times the volume of the clear liquid was added for alcohol precipitation, and stood for 12 hours , and centrifuge again to collect the precipitate. The precipitate was washed three times with 300ml of ethanol and 300ml of acetone in turn, then dissolved in distilled water, and then papain with a weight of 2‰ of the precipitate was added, placed in a water bath at 50°C for 4 hours, and the protein was removed by Sevage method. Add 8g of activated carbon and place it in a 70°C w...
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