Broad-bean chili sauce maturity-promotion and aroma-enhancement fermentation bacterium agent and preparation method thereof
A kind of technology of bean chili sauce and fermented bacterial agent, which is applied to the preparation of the fermented bean curd agent for promoting ripening and increasing aroma of bean chili sauce. Unstable song quality and other problems, to achieve the effect of improving product quality, shortening production cycle, and easy operation
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Embodiment 1
[0025] A kind of preparation method of fermented bacterium agent for promoting ripening and increasing flavor of watercress chili sauce, comprises the steps:
[0026] (1) Propagation and cultivation:
[0027] Bacillus pumilus ( Bacillus pumilus ) ACCC10615 (from China Agricultural Microorganism Culture Collection Management Center) was inoculated in nutrient broth medium at 37°C after being activated and passed the pure inspection, and cultured for 24 hours, the colony count reached 10 6 -10 9 cfu / mL, centrifuge to take the precipitate, and distilled water to elute the precipitate 2-4 times to prepare resting cells;
[0028] Lactobacillus fermentum ( L. fermentum ) CCTCCM87079 (from China Center for Type Culture Collection) and Lactobacillus double fermentum ( L. bifermentans) CGMCC1.1876 (derived from JCM Japan Culture Collection of Microorganisms) was inoculated in MRS liquid medium at 42°C after being activated and purified, and cultured for 36 hours. The colony count...
Embodiment 2
[0039] A kind of preparation method of fermented bacterium agent for promoting ripening and increasing flavor of watercress chili sauce, comprises the steps:
[0040] (1) Propagation and cultivation:
[0041] Bacillus pumilus ( Bacillus pumilus ) ACCC10615 (from China Agricultural Microorganism Culture Collection Management Center) was inoculated in nutrient broth medium at 35°C after being activated and passed the pure inspection, and cultured for 24 hours, and the colony count reached 10 6 -10 9 cfu / mL, centrifuge to take the precipitate, and distilled water to elute the precipitate 2-4 times to prepare resting cells;
[0042] Lactobacillus fermentum ( L. fermentum ) CCTCCM87079 (from China Center for Type Culture Collection) and Lactobacillus double fermentum ( L. bifermentans)CGMCC1.1876 (from JCM Japan Culture Collection of Microorganisms) was inoculated in MRS liquid medium at 40°C after passing the activation and pure test respectively, and cultured for 36 hours, ...
Embodiment 3
[0053] A kind of preparation method of fermented bacterium agent for promoting ripening and increasing flavor of watercress chili sauce, comprises the steps:
[0054] (1) Propagation and cultivation:
[0055] Bacillus pumilus ( Bacillus pumilus ) ACCC10615 (from China Agricultural Microorganism Culture Collection Management Center) was inoculated in nutrient broth medium at 36°C after being activated and passed the pure inspection, and cultured for 24 hours, and the colony count reached 10 6 -10 9 cfu / mL, centrifuge to take the precipitate, and distilled water to elute the precipitate 2-4 times to prepare resting cells;
[0056] Lactobacillus fermentum ( L. fermentum ) CCTCCM87079 (from China Center for Type Culture Collection) and Lactobacillus double fermentum ( L. bifermentans) CGMCC1.1876 (from JCM Japan Culture Collection of Microorganisms) was inoculated in MRS liquid medium at 41°C after passing the activation and pure test respectively, and cultured for 36 hours,...
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