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Microbial fertilizer with high organic boron content and preparation method of microbial fertilizer

A technology of microbial fertilizer and organic boron, which is applied in the field of microbial fertilizer with high organic boron content and its preparation, can solve the problems of product quality and yield decline, grain failure, poor plant growth, etc., and achieve high utilization rate, quick effect, The effect of great application value and economic benefits

Active Publication Date: 2014-10-01
广西多美施肥业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sufficient boron supply leads to luxuriant plant growth, plump grains, good root system, and a good harvest; on the contrary, insufficient boron supply leads to poor plant growth and reduced product quality and yield; when boron is severely deficient, even grains fail

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A preparation method of high organic boron content microbial fertilizer, comprising the steps of:

[0020] (1) Activation of strains:

[0021] Inoculate the slant surface of Bacillus amyloliquefaciens strains stored in the refrigerator on the plate of nutrient broth agar medium, draw a diagonal line, culture in a constant temperature incubator at 30°C for 24 hours, and transfer twice in a row;

[0022] (2) Preparation of seed liquid culture medium:

[0023] The seed liquid culture medium contains 0.5wt% of beef extract, 1wt% of peptone, 0.5wt% of NaCl, and pH7.2. The volume of the seed solution is 50ml in a 250ml Erlenmeyer flask, and it is sterilized at 121°C for 20min. The inoculation amount is 2 rings per bottle. Cultivate in a shaker incubator with a rotation speed of 200r / min and a temperature of 30°C for 28h;

[0024] (3) Preparation of fermentation medium:

[0025] In the fermentation medium, the formula content of each component is: glucose 20g / L, L-sodium ...

Embodiment 2

[0031] A preparation method of high organic boron content microbial fertilizer, comprising the steps of:

[0032] (1) Activation of strains: Inoculate the slant surface of Bacillus amyloliquefaciens strains stored in the refrigerator on the plate of nutrient broth agar medium, draw a diagonal line, cultivate in a constant temperature incubator at 30°C for 24 hours, and transfer twice consecutively ;

[0033] (2) Preparation of seed liquid culture medium:

[0034] The seed liquid culture medium contains beef extract 0.5%, peptone 1%, NaCl 0.5%, pH7.4. The volume of the seed solution is 50ml in a 250ml Erlenmeyer flask, and it is sterilized at 121°C for 20min. The inoculation amount is 2 rings per bottle. Cultivate in a shaker incubator with a rotation speed of 250r / min and a temperature of 33°C for 24h;

[0035] (3) preparation of fermentation medium;

[0036] In the fermentation medium, the formula content of each component is: glucose 30g / L, L-sodium glutamate 10g / L, MgS...

Embodiment 3

[0041] A preparation method of high organic boron content microbial fertilizer, comprising the steps of:

[0042] (1) Activation of strains:

[0043] Inoculate the slant surface of Bacillus amyloliquefaciens strains stored in the refrigerator on the plate of nutrient broth agar medium, draw a diagonal line, cultivate in a constant temperature incubator at 30°C for 26 hours, and transfer twice in a row;

[0044] (2) Preparation of seed liquid culture medium:

[0045] The seed liquid culture medium contains beef extract 0.5%, peptone 1%, NaCl 0.5%, pH7.4. The volume of the seed solution is 50ml in a 250ml Erlenmeyer flask, and it is sterilized at 121°C for 20min. The inoculation volume is 3 rings per bottle. Cultivate in a shaker incubator with a rotation speed of 220r / min and a temperature of 30°C for 26h;

[0046] (3) Preparation of fermentation medium:

[0047] In the fermentation medium, the formula content of each component is: glucose 20g / L, L-sodium glutamate 8g / L, M...

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PUM

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Abstract

The invention discloses a microbial fertilizer with high organic boron content. The fertilizer is prepared through carrying out deep liquid fermentation on bacillus amyloliquefaciens in a high-boron culture medium, wherein the total viable count of the bacillus amyloliquefaciens in the fermentation solution is up to more than 108cfu / ml, and the content of organic boron in the fermentation solution is up to more than 0.04g / L. The invention also provides a preparation method of the microbial fertilizer with high organic boron content. Inorganic boron salt in a raw material culture medium is converted into the organic boron which is easily dissolved into water and easily absorbed and utilized by plants through the deep liquid fermentation of the microbial bacillus amyloliquefaciens. The microbial fertilizer disclosed by the invention has the characteristics of high organic boron content, high biological activity, high utilization ratio, good absorbability, rapid taking effects and the like and can be applied to trees of fruits such as watermelons, strawberries, grapes and peaches.

Description

technical field [0001] The invention relates to the technical field of biological fertilizer production, in particular to a microbial fertilizer with high organic boron content and a preparation method thereof. Background technique [0002] A large number of domestic and foreign scientific experiments have confirmed that boron is involved in many important physiological activities of crops and is an important nutrient element necessary for crop growth and development. For example, boron can promote cell division and elongation, and can inhibit the formation of toxic phenolic compounds; boron can promote nitrogen in plants. Metabolize and improve the root system's need for oxygen; boron can enhance root system function and promote root system development; boron can also improve the viscosity of cell protoplasm and enhance disease resistance. In addition, the normal supply of boron can increase the content of vitamins and sugar in the fruit, reduce the total acid content, and ...

Claims

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Application Information

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IPC IPC(8): C05F11/08C05F17/00
CPCY02W30/40
Inventor 廖祥儒赵晓联黎循航毛飞君周智慧
Owner 广西多美施肥业有限公司