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Method for producing ethanol by virtue of simultaneous saccharification and co-fermentation

A technology of simultaneous saccharification and co-fermentation, applied in microorganism-based methods, biochemical equipment and methods, and fermentation, etc., can solve the problems of uneven mixing, increased inhibitor concentration, and hindered mass transfer, and can solve the problems of uneven mixing. Effect

Inactive Publication Date: 2014-10-01
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to propose a comprehensive use of substrates, enzymes and A method for the production of ethanol by simultaneous saccharification and co-fermentation with high solids content by strain-feeding strategy

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for synchronous saccharification and co-fermentation to produce ethanol, comprising the steps of:

[0024] (1) Soak corn stalks in a stainless steel reactor with a mass ratio of 1:10 in ammonia water with a mass concentration of 25% at 220°C for 20 minutes of soaking pretreatment to obtain a solid-liquid mixture, which is separated and pretreated After the solid, dry;

[0025] (2) Mix the dry solid, cellulolytic enzyme and water in a ratio of 4g:6FPU:80g, adjust the pH to 4.5, and carry out enzymatic hydrolysis for 12 hours;

[0026] (3) Aseptic operation, inoculate Saccharomyces cerevisiae into the mixed solution obtained in step (2) to make the concentration 0.5g / L, carry out synchronous saccharification and co-fermentation, and ferment for 144h at 39°C and 150rpm. Add dry solids, cellulolytic enzymes and Saccharomyces cerevisiae once every 24 hours, add dry solids and cellulolytic enzymes 5 times in total, add Saccharomyces cerevisiae 4 times, add 2 g of dr...

Embodiment 2

[0028] A method for synchronous saccharification and co-fermentation to produce ethanol, comprising the steps of:

[0029] (1) Immerse wheat straw in a stainless steel reactor in ammonia water with a mass concentration of 28% at 160°C for 60 minutes in a mass ratio of 1:5 to obtain a solid-liquid mixture, separate and obtain pretreated After the solid, dry;

[0030] (2) Mix the dry solid, cellulolytic enzyme, and water in a ratio of 2g:2FPU:92g, adjust the pH to 5.5, and carry out enzymatic hydrolysis for 48 hours;

[0031] (3) Aseptic operation, inoculate Saccharomyces cerevisiae into the mixture obtained in step (2) to make the concentration 2g / L, carry out synchronous saccharification and co-fermentation, and ferment for 72 hours at 39°C and 150rpm, with an interval of 12 hours from the beginning of fermentation Add dry solids, cellulolytic enzyme and Saccharomyces cerevisiae once, add dry solids and cellulolytic enzymes twice, add Saccharomyces cerevisiae twice, add dry s...

Embodiment 3

[0033] A method for synchronous saccharification and co-fermentation to produce ethanol, comprising the steps of:

[0034] (1) Immerse wheat straw in a stainless steel reactor in ammonia water with a mass concentration of 28% at 160°C for 60 minutes in a mass ratio of 1:5 to obtain a solid-liquid mixture, separate and obtain pretreated After the solid, dry;

[0035] (2) Mix the dry solid, cellulolytic enzyme and water at a ratio of 2g:2FPU:80g, adjust the pH to 4.5, and carry out enzymatic hydrolysis for 48 hours; starting from the enzymatic hydrolysis, add dry solid once every 24 hours and cellulolytic enzyme, add 1 time, add 2g dry solid and 2FPU cellulolytic enzyme each time;

[0036] (3) Aseptic operation, inoculate Saccharomyces cerevisiae into the mixture obtained in step (2) to make the concentration 2g / L, carry out synchronous saccharification and co-fermentation, and ferment for 72h at 33°C and 150rpm, with an interval of 12h from the beginning of fermentation Add d...

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Abstract

The invention discloses a method for producing ethanol by virtue of simultaneous saccharification and co-fermentation. The method comprises the following steps: (1) soaking straws in ammonia water for pretreatment, to obtain a solid-liquid mixer, separating to obtain a pretreated solid, and drying; (2) mixing the dried solid, cellulose hydrolase and water, regulating pH, carrying out enzymolysis, and adding the dried solid and cellulose hydrolase at the starting of enzymolysis; (3) inoculating brewer yeast to the mixed solution obtained in the step (2), fermenting, and adding the dried solid, the cellulose hydrolase and the brewer yeast at the starting of fermentation, to obtain fermentation liquid rich in ethanol, wherein the preservation number of the brewer yeast is CGMCC No.6634. According to the method, the problem that the mixing is uneven in the simultaneous saccharification and co-fermentation of high-solid content can be effectively solved. The concentration of inhibitors in fermentation of high-solid content can be reduced by virtue of the fed-batch means of strains, the activity of the strains can be improved, and the output of the ethanol can be increased.

Description

technical field [0001] The invention relates to the technical field of biomass new energy, in particular to a method for synchronous saccharification and co-fermentation with high solid content to produce ethanol by comprehensively using substrate, enzyme and bacterial strain feeding strategy. Background technique [0002] At present, the proven reserves of crude oil on the earth have not increased and the output is close to the limit. Human beings are facing the severe challenge of energy and oil crisis. Many countries have begun to formulate or adjust energy policies, and put the research and development of bioenergy in an important position. As a safe and clean fuel and gasoline additive, ethanol has attracted much attention. The traditional ethanol fermentation industry mainly uses grain starch such as corn and wheat or sugarcane juice as raw materials, but the cost of raw materials is as high as 40% of the total cost. However, the current situation of China with a sma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12P19/14C12R1/865
CPCY02E50/10
Inventor 元英进朱家庆李炳志秦磊
Owner TIANJIN UNIV